Title: Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay
Abstract
Cooperativity is an important parameter to understand the ternary complexes formed by protein degraders. We developed fluorine NMR competition binding experiments to determine cooperativity of PROTACs. We show applicability to estimate both positive and negative cooperativity, also with homo-dimerizers, and highlight key features and considerations for optimal assay development.
Two new papers out! Check this out to know more about them! Our new paper ‘Leveraging dual-ligase recruitment to enhance degradation via a heterotrivalent PROTAC’ which spearheaded by former lab…
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