Our collaboration with Dario Alessi’s Lab on deploying improved HaloPROTACs against CRISPR’ed halo-tagged endosomal proteins is now out in ACS Chem Biol

Read the open access article

Congratulations to Hannah Tovell in Dario Alessi’s group for the excellent job on this study. Well done Andrea and Chiara for the chemistry contributions to the project!

TOC graphic for haloPROTACS
TOC graphic for haloPROTACS
Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR/Cas9
Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR/Cas9
Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7
Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7
Figure 3. Chemical structures of HaloPROTAC3 and Compounds A–F
Figure 3. Chemical structures of HaloPROTAC3 and Compounds A–F
Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins
Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins
Figure 5. Mechanistic characterization of HaloPROTAC-E
Figure 5. Mechanistic characterization of HaloPROTAC-E
Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3
Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3
Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins
Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins
Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact
Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact

Related Articles