Publications

For all publications from the Sapkota Lab, please click here.

Individual publications appear below in reverse chronological order.

Bozatzi, P., and Sapkota, G.P. (2018) The FAM83 family of proteins: from pseudo-PLDs to anchors of CK1 isoforms. Biochem Soc Trans,

Fulcher, L. J., Bozatzi, P., Tachie-Menson, T., Cummins, T. D., Wu, K., Dunbar, K., Shrestha, S., Wood, N., Weidlich, S., Macartney, T. J., Varghese, J., Gourlay, R., Campbell, D. G., Dingwell, K. S., Smith, J. C., Bullock, A., and Sapkota, G. P. (2018) The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms. Sci signalling, Vol. 11, Issue 531, eaao2341 DOI: 10.1126/scisignal.aao2341 [Full Text][pdf]

Bozatzi, P., Dingwell, K. S., Wu, K., Cooper, F., Cummins, T. D., Vogt, J., Wood, N., Macartney, T. J., Varghese, J., Gourlay, R., Campbell, D. G., Smith, J. C., and Sapkota, G. P. (2018) PAWS1/FAM83G controls Wnt signalling through association with Casein Kinase 1 alpha. Embo reports, , [Full Text][pdf]

Ramachandran, A., Vizan, P., Das, D., Chakravarty, P., Vogt, J., Rogers, K.W., Müller, P., Hinck, A.P., Sapkota, G.P., and Hill, C.S. (2018) TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition. Elife. Jan 29;7. pii: e31756. doi: 10.7554/eLife.31756. [Full Text] [pdf]

Cummins, T. D., Wu, K. Z. L., Bozatzi, P., Dingwell, K. S., Macartney, T. J., Wood, N. T., Varghese, J., Gourlay, R., Campbell, D. G., Prescott, A., Griffis, E., Smith, J. C., and Sapkota, G. P. (2018) FAM83G/PAWS1 controls cytoskeletal dynamics and cell migration through association with the SH3 adaptor CD2AP. J Cell Sci Jan 10;131(1) [Full Text] [pdf]

Hutchinson, L. D., Bozatzi, P., Macartney, T. J., and Sapkota, G. P. (2018). Generation of endogenous BMP transcriptional reporter cells through CRISPR/Cas9 genome editing. Methods Mol Biol (In Press)

Fernandez-Alonso, R., Davidson, L., Hukelmann, J., Zengerle, M., Prescott, A. R., Lamond, A., Ciulli, A., Sapkota, G. P. and Findlay, G. M. (2017). Brd2-4 functional dynamics coordinate pluripotent exit with Smad2-dependent lineage specification. EMBO Rep Jul;18(7):1108-1122. [Full Text] [pdf]

Cummins T.D., Sapkota, G.P. (2017). Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry. Methods Mol Biol. 2017 Oct 25. [Full Text] [pdf]

Macartney, T.J., Sapkota, G.P. and Fulcher, L.J. (2017) An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins. Vol 7, Iss 22, November 20, 2017 [Full Text] [pdf]

Fulcher, L. J., Macartney, T. J., Turnbull, C., Hutchinson, L., and Sapkota, G. P. (2017) Targeting endogenous proteins for degradation through the affinity-directed protein missile system. Open biology, 7: 170066. [Full Text] [pdf]

Fulcher, L. J., Macartney, T., Bozatzi, P., Hornberger, A., Rojas-Fernandez, A., and Sapkota, G. P. (2016) An affinity-directed protein missile system for targeted proteolysis. Open biology, 6: 160255 [Full Text] [pdf]

Cammareri, P., Rose, A. M., Vincent, D. F., Wang, J., Nagano, A., Libertini, S., Ridgway, R. A., Athineos, D., Coates, P. J., McHugh, A., Pourreyron, C., Dayal, J. H., Larsson, J., Weidlich, S., Spender, L. C., Sapkota, G. P., Purdie, K. J., Proby, C. M., Harwood, C. A., Leigh, I. M., Clevers, H., Barker, N., Karlsson, S., Pritchard, C., Marais, R., Chelala, C., South, A. P., Sansom, O. J. and Inman, G. J. (2016). Inactivation of TGFbeta receptors in stem cells drives cutaneous squamous cell carcinoma. Nat Commun 7, pp. 12493 [Full Text] [pdf]

Rojas-Fernandez, A., Herhaus, L., Macartney, T., Lachaud, C., Hay, R. T., and Sapkota, G. P. (2015) Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Sci Rep 5, 9811 [Full Text] [pdf]

Herhaus, L., Perez-Oliva, A. B., Cozza, G., Gourlay, R., Weidlich, S., Campbell, D. G., Pinna, L. A., and Sapkota, G. P. (2015) Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal 8, ra35 [Full Text] [pdf]

Tan, L., Nomanbhoy, T., Gurbani, D., Patricelli, M., Hunter, J., Geng, J., Herhaus, L., Zhang, J., Pauls, E., Ham, Y., Choi, H. G., Xie, T., Deng, X., Buhrlage, S. J., Sim, T., Cohen, P., Sapkota, G. P., Westover, K. D. and Gray, N. S. (2015) Discovery of type II inhibitors of TGFβ-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). J Med Chem. 2015 Jan 8;58(1):183-96. doi: 10.1021/jm500480k. [Full Text] [pdf]

Herhaus, L., and Sapkota, G. P. (2014) The emerging roles of deubiquitylating enzymes (DUBs) in the TGFbeta and BMP pathways. Cell Signal 26, 2186-2192 [Full Text] [pdf]

Herhaus, L., Al-Salihi, M. A., Dingwell, K. S., Cummins, T. D., Wasmus, L., Vogt, J., Ewan, R., Bruce, D., Macartney, T., Weidlich, S., Smith, J. C., and Sapkota, G. P. (2014) USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling. Open biology 4, 140065 [Full Text] [pdf]

Vogt, J., Dingwell, K. S., Herhaus, L., Gourlay, R., Macartney, T., Campbell, D., Smith, J. C., and Sapkota, G. P. (2014) Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling. Open biology 4, 130210 [Full Text] [pdf]

Herhaus, L., Al-Salihi, M., Macartney, T., Weidlich, S., and Sapkota, G. P. (2013) OTUB1 enhances TGFbeta signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nature communications 4, 2519 [Full Text] [pdf]

Sapkota, G. P. (2013) The TGF-beta-induced phosphorylation and activation of p38 mitogen-activated protein kinase is mediated by MAP3K4 and MAP3K10 but not TAK1. Open biology 3, 130067 [Full Text] [pdf]

Bruce, D. L. and Sapkota, G. P. (2012). Phosphatases in SMAD regulation. FEBS letters 586, pp. 1897-1905 [Full Text] [pdf]

Bruce, D. L., Macartney, T., Yong, W., Shou, W., and Sapkota, G. P. (2012) Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. Cell Signal 24, 1999-2006 [Full Text] [pdf]

Al-Salihi, M. A., Herhaus, L., and Sapkota, G. P. (2012) Regulation of the transforming growth factor beta pathway by reversible ubiquitylation. Open biology 2, 120082 [Full Text] [pdf]

Al-Salihi, M. A., Herhaus, L., Macartney, T., and Sapkota, G. P. (2012) USP11 augments TGFbeta signalling by deubiquitylating ALK5. Open biology 2, 120063 [Full Text] [pdf]

Vogt, J., Traynor, R. and Sapkota, G. P. (2011). The specificities of small molecule inhibitors of the TGFß and BMP pathways. Cell Signal 23, pp. 1831-1842 [Full Text] [pdf]

Other Prior Publications From G. Sapkota

Alarcon, C., Zaromytidou, A. I., Xi, Q., Gao, S., Yu, J., Fujisawa, S., Barlas, A., Miller, A. N., Manova-Todorova, K., Macias, M. J., Sapkota, G., Pan, D. and Massague, J. (2009). Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Cell 139, pp. 757-69 [Full Text] [pdf]

Gao, S., Alarcon, C., Sapkota, G., Rahman, S., Chen, P. Y., Goerner, N., Macias, M. J., Erdjument-Bromage, H., Tempst, P. and Massague, J. (2009). Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell 36, pp. 457-68 [Full Text] [pdf]

Sapkota, G. P., Cummings, L., Newell, F. S., Armstrong, C., Bain, J., Frodin, M., Grauert, M., Hoffmann, M., Schnapp, G., Steegmaier, M., Cohen, P. and Alessi, D. R. (2007). BI-D1870 is a specific inhibitor of the p90 RSK (ribosomal S6 kinase) isoforms in vitro and in vivo. Biochem J 401, pp. 29-38 [Full Text] [pdf]

Sapkota, G., Alarcon, C., Spagnoli, F. M., Brivanlou, A. H. and Massague, J. (2007). Balancing BMP signaling through integrated inputs into the Smad1 linker. Mol Cell 25, pp. 441-54 [Full Text] [pdf]

Knockaert, M.†, Sapkota, G.†, Alarcón, C., Massagué, J. and Brivanlou, A.H. (2006) Unique players in the BMP pathway: small C-terminal domain phosphatases dephosphorylate Smad1 to attenuate BMP signaling. [†equal contribution] Proc. Nat. Acad. of Sci. USA. 103, 11940-5 [Full Text] [pdf]

Sapkota, G., Knockaert, M., Alarcon, C., Montalvo, E., Brivanlou, A. H. and Massague, J. (2006). Dephosphorylation of the linker regions of Smad1 and Smad2/3 by small C-terminal domain phosphatases has distinct outcomes for bone morphogenetic protein and transforming growth factor-beta pathways. J Biol Chem 281, pp. 40412-9 [Full Text] [pdf]

Morgan, C. P., Skippen, A., Segui, B., Ball, A., Allen-Baume, V., Larijani, B., Murray-Rust, J., McDonald, N., Sapkota, G., Morrice, N. and Cockcroft, S. (2004). Phosphorylation of a distinct structural form of phosphatidylinositol transfer protein alpha at Ser166 by protein kinase C disrupts receptor-mediated phospholipase C signaling by inhibiting delivery of phosphatidylinositol to membranes. J Biol Chem 279, pp. 47159-71 [Full Text] [pdf]

Baas, A. F., Boudeau, J., Sapkota, G. P., Smit, L., Medema, R., Morrice, N. A., Alessi, D. R. and Clevers, H. C. (2003). Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD. EMBO J 22, pp. 3062-72 [Full Text] [pdf]

Boudeau, J., Sapkota, G. and Alessi, D. R. (2003). LKB1, a protein kinase regulating cell proliferation and polarity. FEBS Lett 546, pp. 159-65 [Full Text] [pdf]

Douglas, P., Sapkota, G. P., Morrice, N., Yu, Y., Goodarzi, A. A., Merkle, D., Meek, K., Alessi, D. R. and Lees-Miller, S. P. (2002). Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase. Biochem J 368, pp. 243-51 [Full Text] [pdf]

Sapkota, G. P., Boudeau, J., Deak, M., Kieloch, A., Morrice, N. and Alessi, D. R. (2002). Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem J 362, pp. 481-90 [Full Text] [pdf]

Sapkota, G. P., Deak, M., Kieloch, A., Morrice, N., Goodarzi, A. A., Smythe, C., Shiloh, Y., Lees-Miller, S. P. and Alessi, D. R. (2002). Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366. Biochem J 368, pp. 507-16 [Full Text] [pdf]

Lee, M. J., Thangada, S., Paik, J. H., Sapkota, G. P., Ancellin, N., Chae, S. S., Wu, M., Morales-Ruiz, M., Sessa, W. C., Alessi, D. R. and Hla, T. (2001). Akt-mediated phosphorylation of the G protein-coupled receptor EDG-1 is required for endothelial cell chemotaxis. Mol Cell 8, pp. 693-704 [Full Text] [pdf]

Sapkota, G. P., Kieloch, A., Lizcano, J. M., Lain, S., Arthur, J. S., Williams, M. R., Morrice, N., Deak, M. and Alessi, D. R. (2001). Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth. J Biol Chem 276, pp. 19469-82 [Full Text] [pdf]

Godber, B. L., Doel, J. J., Sapkota, G. P., Blake, D. R., Stevens, C. R., Eisenthal, R. and Harrison, R. (2000). Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase. J Biol Chem 275, pp. 7757-63 [Full Text] [pdf]