Tagging
| Plasmid | Map (PDF) | Sequence | Description |
|---|---|---|---|
| pRPaTAG | pRPaTAG map | pRPa sequence | Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA spacer locus in T. brucei.Variants available (HYG): pRPaiGFPx, pRPaixGFP, pRPaiMYCx, pRPaix6MYCxConstitutive expression plasmids, pRPaGFPx and pRPax6MYCx, are also available pRPaTAG Details (PDF) |
| pRPTAG | pRPTAG map | PRP sequence | Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally tagged proteins from a random RRNA spacer locus in T. brucei.Variants available (HYG): pRPiGFPx, pRPiMYCx (also BSD), pRPix6MYCx (also BSD)Constitutive expression plasmids (BSD), pRPxGFP and pRPx6MYCx, are also available |
| pT7TAG | pT7TAG map | pT7 sequence | Tet-inducible T7 promoter driven plasmid for inducible expression of N-terminally tagged proteins from a random RRNA spacer in T. brucei.Variants available (HYG): pT7iGFPx, pRPiMYCxpT7TAG Details (PDF) |
| pNATTAGx | pNATTAGx map | pNATTAGx sequence | A plasmid for expression of N-terminally tagged proteins from a gene’s native locus.Variants available (BSD and HYG): pNATGFPx, pNAT6MYCx |
| pNATxTAG | pNATxTAG map | pNATxTAG sequence | A plasmid for expression of C-terminally tagged proteins from a gene’s native locus.Variants available (BSD and HYG): pNATxGFP, pNATx12MYC |
RNAi
| Plasmid | Map (PDF) | Sequence | Description |
|---|---|---|---|
| pRPaiSL | pRPaSL map | pRPa sequence | Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.pRPaSL Details (PDF) |
| p2T7TAblue | p2T7TAblue map | p2T7TAblue sequence | Head-to-head T7 promoter-driven plasmid for the generation of dsRNA for RNAi experiments in T. brucei.p2T7aTAblue (sequence) is also available – compatible with the tagged locus cell line when modified to express T7 polymerase.Details (PDF) |
Plasmids
Several of these constructs are also available from Addgene.
View inducible expression data.
For more information on cells and vectors see: Inducible cell lines (word document), and George Cross and Christine Clayton’s web-sites.