pNATBSD N-terminal in situ tagging construct

pNATBSD N-terminal in situ tagging construct

Derived from known sequence

Landmarks Available expression cassettes
0001-2497: (2497) Vector backbone Sequence page Expression cassette Region replaced
2498-2736: (239) T. brucei βα tubulin mRNA processing 5′ N-terminal eGFP GFPx HindIII – BamHI
2747-3145: (399) Blasticidin-S deaminase ORF
3161-3483: (323) T. brucei αβ tubulin mRNA processing 3′ Additional selectable markers
3484-3728: (245) 6MYC Expression Cassette HYG 6MYCx SpeI – SacI
HindIII PacI ATG XbaI — [cMYC]6AvrII TGA BamHI GFPx
    1  GAATTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
   51  ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
  101  TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
  151  CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
  201  CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
  251  GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
  301  GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
  351  TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
  401  CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
  451  GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
  501  GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
  551  TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
  601  GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
  651  AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
  701  CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
  751  CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
  801  CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
  851  TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
  901  TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
  951  TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
 1001  TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
 1051  GTTCATCCAT AGTTGCCTGA CTCCCCGTGG TGTAGATAAC TACGATACGG
 1101  GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
 1151  CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
 1201  AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
 1251  TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
 1301  CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
 1351  TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
 1401  CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
 1451  CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
 1501  ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
 1551  GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
 1601  CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
 1651  TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
 1701  ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
 1751  CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
 1801  CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
 1851  TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
 1901  TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
 1951  AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA
 2001  GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
 2051  GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA
 2101  TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
 2151  AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG
 2201  GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATTCGA
 2251  CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT
 2301  GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCAAGG AGATGGCGCC
 2351  CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG
 2401  CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG
 2451  GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCTGG
 2501  GTCCCATTGT TTGCCTCTTC CGCTGTGTGG AGTGCGCCTA CACGCACTTC
 2551  TCACTTCGTA AGTGGTGGTG GCGTAAGTAT TGCCTAATGT TGACTCTATG
 2601  TTCTCCTCTC CTCACCCCCT CGCGGTGCTG ATTTCTGACA GATCTTCAAA
 2651  CACTAGTTTA AGCAAAGGAC TATTCATCCG TTTATATTAG CAACAGTAGG
 2701  TACTAGCACC ACTAACAACA ACAACAAAGC ACTTCTGCAG GAATTCATGG
 2751  CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT
 2801  ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC
 2851  TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA
 2901  CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
 2951  GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG
 3001  CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC
 3051  CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT
 3101  GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAGAATT
 3151  CGAGCTCGCC CTAGAAAGTG TGACAACGTC GCACCATGTG TAGGTTTTCA
 3201  TTTATGTTCT TTCTTTCTTT CTTTTGTGAA TTTGTTTTCT GTCTCAAATG
 3251  TTTTTAATTC GCTTGGGACC TATGTTTTTC TTGTTTTTTT GCTCACCCTT
 3301  TGTGTAGGAG GCACCCTGTC ACGTCTGTGG TTGCGTGTAT GCCTTCCTTC
 3351  CCCTTATTCG CTTCTTCCTG TCGTGTCACA CCTCTTTCTC CCTCTCCCTT
 3401  TCCGCCTTTT CTTTCAATCT TGTTTTCTCG ACCAGCCCTA CTAGAGGAGA
 3451  AAGAATAGTA ACCCTTTCAT CAAAGAAAAT AGTAAGCTTA ATTAATGTCT
 3501  AGAGAACAGA AACTGATCTC TGAAGAAGAC CTGCCTAGAG AACAGAAACT
 3551  GATCTCTGAA GAAGACCTGC CTAGAGAACA GAAACTGATC TCTGAAGAAG
 3601  ACCTGCCTAG AGAACAGAAA CTGATCTCTG AAGAAGACCT GCCTAGAGAA
 3651  CAGAAACTGA TCTCTGAAGA AGACCTGCCT AGAGAACAGA AACTGATCTC
 3701  TGAAGAAGAC CTGCCTAGGT GAGGATCC