Resources

Resources

A list of available resources

Plasmid	Map (PDF)	Sequence	Description
pRPa^TAG	pRPa^TAG map	pRPa sequence	Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA spacer locus in T. brucei. Variants available (HYG): pRPa^iGFPx, pRPa^ixGFP, pRPa^iMYCx, pRPa^ix6MYCx Constitutive expression plasmids, pRPa^GFPx and pRPa^x6MYCx, are also available pRPa^TAG Details (PDF)
pRP^TAG	pRP^TAG map	PRP sequence	Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally tagged proteins from a random RRNA spacer locus in T. brucei. Variants available (HYG): pRP^iGFPx, pRP^iMYCx (also BSD), pRP^ix6MYCx (also BSD) Constitutive expression plasmids (BSD), pRP^xGFP and pRP^x6MYCx, are also available
pT7^TAG	pT7^TAG map	pT7 sequence	Tet-inducible T7 promoter driven plasmid for inducible expression of N-terminally tagged proteins from a random RRNA spacer in T. brucei. Variants available (HYG): pT7^iGFPx, pRP^iMYCx pT7^TAG Details (PDF)
pNAT^TAGx	pNAT^TAGx map	pNAT^TAGx sequence	A plasmid for expression of N-terminally tagged proteins from a gene’s native locus. Variants available (BSD and HYG): pNAT^GFPx, pNAT^6MYCx
pNATx^TAG	pNATx^TAG map	pNATx^TAG sequence	A plasmid for expression of C-terminally tagged proteins from a gene’s native locus. Variants available (BSD and HYG): pNAT^xGFP, pNAT^x12MYC

RNAi

Plasmid	Map (PDF)	Sequence	Description
pRPa^iSL	pRPaSL map	pRPa sequence	Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei. pRPaSL Details (PDF)
p2T7^TAblue	p2T7^TAblue map	p2T7^TAblue sequence	Head-to-head T7 promoter-driven plasmid for the generation of dsRNA for RNAi experiments in T. brucei. p2T7aTAblue (sequence) is also available – compatible with the tagged locus cell line when modified to express T7 polymerase. Details (PDF)

Plasmid

Map (PDF)

Sequence

Description

pRPa^iSL

pRPaSL map

pRPa sequence

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

p2T7^TAblue

p2T7^TAblue map

p2T7^TAblue sequence

Head-to-head T7 promoter-driven plasmid for the generation of dsRNA for RNAi experiments in T. brucei.

p2T7aTAblue (sequence) is also available – compatible with the tagged locus cell line when modified to express T7 polymerase.

Several of these constructs are also available from Addgene.

For more information on cells and vectors see: Inducible cell lines (word document), and George Cross and Christine Clayton’s web-sites.