Search Results for

2016 – 2013

2016 Dr Sebastian Hutchinson 25 November 2016Congratulations to Seb on his Ph.D. award (and Roux Fellowship award) and many thanks to his examiners, Mark Carrington and Tom Owen-Hughes. Biology of Parasitism 5 August 2016David and Seb teach their first module…

2019 – 2017

2019 Trager award 23 November 2019David receives William Trager award for basic parasitology: see WCAIR news, SLS news and UoD news. New funding 11 September 2019Wellcome Trust Investigator Award funding to support the Horn-lab: see SLS news and WCAIR news. Allelic exclusion 9 July 2019Joana’s work reveals…

Links

T. brucei labs >120 (*=retired) Bioinformatics Sequence analysis: Charts: Datab: Other: Journals Review Journals

Blasticidin S Deaminase cassette

Cassette sequenced in full KpnI — START — BSD — STOP — EcoRI NheI 1 GGTACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG 51 AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG 101 CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA 151 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC 201 TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG…

C terminal 12MYC tagging cassette

Cassette sequenced in full x12MYC XbaI — [cMYC]12 — AvrII STOP BamHI 1 TCTAGAGAAC AGAAACTGAT CTCTGAAGAA GACCTGCCTA GAGAACAGAA 51 ACTGATCTCT GAAGAAGACC TGCCTAGAGA ACAGAAACTG ATCTCTGAAG 101 AAGACCTGCC TAGAGAACAG AAACTGATCT CTGAAGAAGA CCTGCCTAGA 151 GAACAGAAAC TGATCTCTGA AGAAGACCTG CCTAGAGAAC AGAAACTGAT 201 CTCTGAAGAA GACCTGCCTA GAGAACAGAA…

Hygromycin b phosphotransferase

Derived from known sequence SpeI — HYG — SacI 1 ACTAGTGATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC 51 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC 101 GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT 151 GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC 201 GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG 251 GAATTCAGCG AGAGCCTGAC CTATTGCATC…

BSD

NgoMIV — αβ-Tubulin intergenic region — STOP BSD START — BstZ17I 1 GCCGGCCCCC TCGACTATTT TCTTTGATGA AAGGGTTACT ATTCTTTCTC 51 CTCTAGTAGG GCTGGTCGAG AAAACAAGAT TGAAAGAAAA GGCGGAAAGG 101 GAGAGGGAGA AAGAGGTGTG ACACGACAGG AAGAAGCGAA TAAGGGGAAG 151 GAAGGCATAC ACGCAACCAC AGACGTGACA GGGTGCCTCC TACACAAAGG 201 GTGAGCAAAA AAACAAGAAA AACATAGGTC CCAAGCGAAT…

N-term tdTomato

TOMx HindIII — START tdTomato — XbaI — 1998 bp stuffer — BamHI1 AAGCTTATGG TGAGCAAGGG CGAGGAGGTC ATCAAAGAGT TCATGCGCTT51 CAAGGTGCGC ATGGAGGGCT CCATGAACGG CCACGAGTTC GAGATCGAGG101 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG151 GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA201 GTTCATGTAC GGCTCCAAGG CGTACGTGAA GCACCCCGCC GACATCCCCG251 ATTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG301 AACTTCGAGG ACGGCGGTCT…

Constitutive

Cassette sequenced in full NheI — RRNA promoter — KpnI 1 GCTAGCTTTC CACCCAGCGC GGGTGCATTC TGGCTCTTAT ATATACTTAT 51 TGTCATGACA GAGTATATTG TACTGTGTTG ATAAGGGACG GGTAACTGTA 101 TTGAAGAGCC GATGCTTTTG ACATGTTAGA TATAATATGT TTTATTGTAA 151 AGTCAATACA ACACACAATA GGATAATAAT GATAAAGTTA AAAAAGTATA 201 TATAGTAATA GAAATATATC TTATATAGGA AAGATTAAGC AGTAAAAGTA…

N/C-term 6MYC

Cassette sequenced in full x6MYCx HindIII PacI START XbaI — [cMYC]6 — AvrII STOP BamHI 1 AAGCTTAATT AATGTCTAGA GAACAGAAAC TGATCTCTGA AGAAGACCTG 51 CCTAGAGAAC AGAAACTGAT CTCTGAAGAA GACCTGCCTA GAGAACAGAA 101 ACTGATCTCT GAAGAAGACC TGCCTAGAGA ACAGAAACTG ATCTCTGAAG 151 AAGACCTGCC TAGAGAACAG AAACTGATCT CTGAAGAAGA CCTGCCTAGA 201…

N-term MYC

Cassette sequenced in full MYCx HindIII — START cMYC — XbaI — 732 or 1,053 bp stuffer — BamHI 1 AAGCTTATGG AACAGAAACT GATCTCTGAA GAAGACCTGT CTAGANNNNN 51 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 151 NNNNNNNNNN NNNNNNNNNN…

C-term eGFP

Cassette sequenced in full xGFP in pRPa PacI HindIII — 389 bp stuffer — XbaI — eGFP STOP — BamHI 1 TTAATTAAGC TTNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 51 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 151 NNNNNNNNNN…

N-term eGFP

Cassette sequenced in full GFPx HindIII — START eGFP — XbaI — 732 or 1812 bp stuffer — BamHI 1 AAGCTTATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT 51 CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 101 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC 151 TGCACCACCG GCAAGCTGCC…

Stem loop RNAi cassettes

Cassette sequenced in full, February 2008 Two versions are available, containing different multiple cloning sites separated by a 468 bp stuffer (pBluescript 117-585, including 125 bp of the β-galactosidase α-fragment) All of the restriction sites listed are unique. MCS1/2 KpnI…

Inducible expression data (bloodstream form T. brucei)

Time Course (hrs)– cells were incubated with 1μg ml-1 tetracycline Dose Response– ng ml-1 tetracycline, 24h Whole cell lysates from cultured Single Marker bloodstream form T. brucei expressing a cMyc-tagged protein (pT7TAG integrated at a RRNA spacer locus) were frationated by SDS-PAGE, transferred to…

p2T7aTAblue sequence

Derived from known sequence and junction sequencing, February 2008 Landmarks 0001-0600: (600) RRNA targeting fragment AscI 0601-3124: (2525) Vector backbone (pUC/pGEM) 3125-3186: (62) Remnant of T. brucei actin mRNA processing 3’ 3187-4207: (1021) Hygromycin B phosphotransferase ORF ΔSTOP AscI 4208-4339: (132) T. brucei actin…

p2T7TAblue sequence

in silico assembly, October 2005 Landmarks 0001-0592: (592) RRNA spacer targeting fragment Not “I” 0593-3116: (2524) Vector backbone (pUC/pGEM) 3117-3412: (296) T. brucei actin mRNA processing 3’ 3413-4435: (1023) Hygromycin B phosphotransferase ORF 4436-4570: (135) T. brucei actin mRNA processing 5’ 4571-4865: (295) RRNA promoter 4866-5338:…

pNATHYG C-terminal in situ tagging construct

Derived from known sequence Landmarks Available expression cassettes 0001-1535: (1535) eGFP Expression cassette Sequence page Expression cassette Region replaced AscI HindIII — 792bp stuffer — XbaI — eGFP — AvrII TGA BamHI C-terminal 12MYC x12MYC XbaI – BamHI 1536-2166: (631) T. brucei βα tubulin mRNA processing 5′ 2180-3205: (1026)…

pNATBSD N-terminal in situ tagging construct

pNATBSD N-terminal in situ tagging construct Derived from known sequence Landmarks Available expression cassettes 0001-2497: (2497) Vector backbone Sequence page Expression cassette Region replaced 2498-2736: (239) T. brucei βα tubulin mRNA processing 5′ N-terminal eGFP GFPx HindIII – BamHI 2747-3145: (399) Blasticidin-S deaminase…

PT7 sequence

Landmarks Available expression cassettes 0001-0592: (592) RRNA targeting fragment NotI Sequence page Expression cassette Region replaced 0593-3116: (2525) Vector backbone (pUC/pGEM) N-term eGFP GFPx HindIII – BamHI 3117-3412: (296) T. brucei actin mRNA processing 3’ N-term MYC MYCx 3413-4435: (1023) Hygromycin B phosphotransferase ORF…

PRP sequence

Landmarks Available expression cassettes 0001-0592: (592) RRNA targeting fragment NotI Sequence page Expression cassette Region replaced 0593-3116: (2525) Vector backbone (pUC/pGEM) N-term eGFP GFPx HindIII – BamHI 3117-3412: (296) T. brucei actin mRNA processing 3’ N-term MYC MYCx 3413-4435: (1023) Hygromycin B phosphotransferase ORF…

pRPa sequence

Landmarks Available expression cassettes 0001-0600: (600) RRNA targeting fragment AscI Sequence page Expression cassette Region replaced 0601-3124: (2525) Vector backbone (pUC/pGEM) Stem loop RNAi MCS1/2 KpnI – Bsp120I 3125-3186: (62) Remnant of T. brucei actin mRNA processing 3’ MCS3/4 3187-4207: (1021) Hygromycin B phosphotransferase ORF…

2014 – 2011

May 2014 Human serum RNAi screen published in PLoS Pathogens. September 2012 Dave promoted to Professor of Molecular Parasitology June 2012 Drug cross-resistance work published in PNAS and featured in: June 2012 Sam Alsford awarded Wellcome Trust Institutional Strategic Support Fund Fellowship January…

Resources

Tagging Plasmid Map (PDF) Sequence Description pRPaTAG pRPaTAG map pRPa sequence Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA spacer locus in T. brucei.Variants available (HYG): pRPaiGFPx, pRPaixGFP, pRPaiMYCx, pRPaix6MYCxConstitutive expression plasmids, pRPaGFPx and pRPax6MYCx, are…

Projects

The African trypanosome (Trypanosoma brucei) diverged early from the eukaryotic lineage and is an important parasite of humans and their livestock. Many fundamental molecular and biochemical processes were first discovered in trypanosomes, some ‘unusual’, others broadly conserved.  An excellent range…

People

David Horn Professor of Molecular Parasitology and Head of Lab Anna Trenaman Post-doc Dr Simone Altmann Post-doc Michele Tinti Post-doc (0.5 FTE) Dr Rupa Nagar Post-doc Dr Douglas Escrivani De Oliveira Post-doc Gustavo Bravo Ruiz Post-doc Marketa Novotna PhD student

News

2025 Rupa Nagar – Mass spectrometry lead November 2025 Rupa joined the team as a post-doc in May and, following excellent work on multiple projects, has now taken up a Mass Spectrometry lead position in Glasgow with Entourage Diagnostics. RNA-based VSG…

Life Sciences building on main campus

David Horn lab homepage

In the Horn lab, our research focuses on antigenic variation, drug action and resistance and the application of genetic screens to the African trypanosome, Trypanosoma brucei

Antigenic variation study

[flyntTheContent id=”0″] Work from Douglas, Viktor & team shows how trypanosomes deploy VSGs economically. Read the full article available on the journal website PLoS Pathogens.

SLS Innovator Award

[flyntTheContent id=”0″] Simone, Sandra, Melanie, Anna, Michele, and Susan win Innovator Award for Oligo Targeting.