| Landmarks | Available expression cassettes | |||
| 0001-0600: (600) | RRNA targeting fragment AscI | Sequence page | Expression cassette | Region replaced |
| 0601-3124: (2525) | Vector backbone (pUC/pGEM) | Stem loop RNAi | MCS1/2 | KpnI – Bsp120I |
| 3125-3186: (62) | Remnant of T. brucei actin mRNA processing 3’ | MCS3/4 | ||
| 3187-4207: (1021) | Hygromycin B phosphotransferase ORF ΔSTOP AscI | |||
| 4208-4465: (258) | T. brucei aldolase mRNA processing 5’ | N-term eGFP | GFPx | HindIII – BamHI |
| 4449-4682: (234) | EP procyclin promoter | C-term eGFP | xGFP | |
| 4688-4947: (260) | RRNA promoter | N-term MYC | MYCx | |
| 4951-4963: (13) | Tet operator | N/C-term 6MYC | x6MYCx | |
| 4970-5064: (95) | T. brucei procyclin mRNA processing 5′ | |||
| 5062-7596: (2535) | Expression Cassette (GFPx = 2,535 bp) | Constitutive | GFPx | NheI – KpnI |
| 7597-8334: (738) | T. brucei aldolase mRNA processing 3’ | |||
| 8335-8736: (402) | T7 terminator x2 fragment | |||
1 GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT
51 CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT
101 TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT
151 AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA
201 CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA
251 TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG
301 GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT
351 GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA
401 GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG
451 GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC
501 TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA
551 GTCTGGAATT AGAATACATA CCAACTATAT GGCGCGCCGA GCTCGAATTC
601 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
651 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
751 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
801 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
851 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
901 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
951 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
1001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
1051 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
1101 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
1151 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
1201 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
1251 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
1301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
1351 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
1401 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
1451 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
1501 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
1551 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
1601 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
1651 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
1701 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
1751 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
1801 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
1851 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
1901 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
1951 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
2001 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
2051 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
2101 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
2151 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
2201 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
2251 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
2301 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
2351 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
2401 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
2451 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
2501 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
2551 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC
2601 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
2651 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
2701 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
2751 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
2801 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT TCGACGCTCT
2851 CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC
2901 GTTGAGCACC GCCGCCGCAA GGAATGGTGC AAGGAGATGG CGCCCAACAG
2951 TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA
3001 TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA
3051 TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG
3101 TCCGGCGTAG AGGATCTGGC TAGGAATACT GCATAGATAA CAAACGCATC
3151 AAACACAACT GGTGGCACTA TTTCAATCAT GTCGACGGCG CGCCCTCGGA
3201 CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC
3251 ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG
3301 TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT
3351 GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC
3401 AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT
3451 GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC
3501 CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC
3551 CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT
3601 TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC
3651 TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC
3701 GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG
3751 CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG
3801 AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
3851 CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA
3901 GGTCTTGCGT GACACCCTGT GCACGGCGGG AGATGCAATA GGTCAGGCTC
3951 TCGCTGAATT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA
4001 TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC
4051 TATTTACCCG CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA
4101 CGAGATTCTT CGCCCTCCGA GAGCTGCATC AGGTCGGAGA CGCTGTCGAA
4151 CTTTTCGATC AGAAACTTCT CGACAGACGT CGCGGTGAGT TCAGGCTTTT
4201 TCATCACTAG ACTTCGTTGC AGTTGAGTTT CTGTTTTCGG GCTTGTTGGT
4251 ATACTTGTTT GATTAATGTG GCTGATAGCT AAGCTATCGC AAATATTTGT
4301 ACAAGACATG AGGGGGCAAA AAAATAAGCC AAGTGTATTT GTTAGGCTTC
4351 CGTGCCTGTT AGAGAAATAT GTAAGGAAAC GCGTGCGCTA CACAGCTTGA
4401 GCACCCATCG AATTGGGACG AGAACATGTT AAACTCAGTA CTCACCCGAT
4451 AACCCCTATT TTCAATGCAC GCCCACCCAG AGACACGGAA TAGCATAAAA
4501 CAACCCCATC ACTGCCACAA AATGGACATT TTGCACAAAA CGCACTATTG
4551 ACTAGTATTC GACTATACTA ATAAGAGATA CCTATCAGAC ACGATATACT
4601 GTGAAAAGGA CATTAAGCTC ACTATGCATC TGAAAACGCT TTCACCTCAC
4651 AACCTACTTA CACACACCTT TCCGCTTAAC CCGCTAGCTT TCCACCCAGC
4701 GCGGGTGCAT TCTGGCTCTT ATATATACTT ATTGTCATGA CAGAGTATAT
4751 TGTACTGTGT TGATAAGGGA CGGGTAACTG TATTGAAGAG CCGATGCTTT
4801 TGACATGTTA GATATAATAT GTTTTATTGT AAAGTCAATA CAACACACAA
4851 TAGGATAATA ATGATAAAGT TAAAAAAGTA TATATAGTAA TAGAAATATA
4901 TCTTATATAG GAAAGATTAA GCAGTAAAAG TAGCGCTTAC GGCGTACACC
4951 CTATCAATGA TAGAGTGGTA CCCTGCACGC GCCTTCGAGT TTTTTTTCCT
5001 TTTCCCCATT TTTTTCAACT TGAAGACTTC AATTACACCA AAAAGTAAAA
5051 TTCACAAGCT TNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5801 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5851 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5901 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5951 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6001 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6051 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6801 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6851 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6901 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6951 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7001 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7051 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
7551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGAT
7601 CCTGCCCATT TAGTTGGCTT TTCCCTTGTC TCGTGTCTTT TCCGTGGAAA
7651 GGTTCCCGGA GTAATCTGAT GGCACAGCAG GGAGGTGCGC CTGCAGGTTG
7701 GTTAGGAAGG GGGGATGATG TAAAAGAAGA AAATGGGGGG ATATCTTATG
7751 TTTAAGAGGA TAAATAATGT GAAGGGGCTT TATATGCTTG CTTGGTTGTC
7801 TCGTTGGTGC ATGGGGAATC TGCATGTTTG CTTTGGAGCA CGCGTGGTAC
7851 ATCAATGGGA TCATATCTTG CTGCCTCCCG CAGCTCACCG TGCGAGCTGC
7901 CGGGACCCCG TTTCTCATAG GCGTGTGCAC CTTCCGTCTG AGACCTGTAA
7951 ATGGTTAAAA AGGAATATAT AATTACCTTT TGAAAAGTGG TAAAAAACGA
8001 ATATATCTTT TTTTTTGGTT TTAATACGCT CTTTTGTGTG TATGAGAGGA
8051 ATAAATAAGT GTGTGTGTGT GTGTGTGTTG TTGTTGTTGA AAAAATGAAG
8101 GACACGAAAG CGCTCATTAA GGGTGGGGAT ACACCAGGCA TAATTATCCG
8151 AAAATATTTT GATGCACCAA ATAAGTGAAC ATACTGACGA AATCAAAGAG
8201 TTGGAGGATG GATAGGGAGG CCTCATTGGC AGTGGTAAAT TGATTTGACT
8251 TGAATATGAT ACCCTCTTAC TGTTTCATTG CCACTACAAG TTGGTTTCCT
8301 TCCCCTGCAG GCATGCAAGC TAGGCCACAC CTATCAGCCG GTGTGTTGCC
8351 AAAGACATTC TGTATAATTT TCCAACTACC CTAACCTGAT TCACTCATCG
8401 TTGAGCATTT GTTCTTCTAC TTTTTGACCG CAAGTGTCGT TATTAGTATT
8451 CGGTCCTGGG CTGCAGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG
8501 CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
8551 CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GAACTATATC CGGATAGGGC
8601 TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC
8651 AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
8701 TTGCTGAAAG GAGAACTATA TCCGGATTGG GCCCCTGCAG