PRP sequence
| Landmarks | Available expression cassettes | |||
| 0001-0592: (592) | RRNA targeting fragment NotI | Sequence page | Expression cassette | Region replaced |
| 0593-3116: (2525) | Vector backbone (pUC/pGEM) | N-term eGFP | GFPx | HindIII – BamHI |
| 3117-3412: (296) | T. brucei actin mRNA processing 3’ | N-term MYC | MYCx | |
| 3413-4435: (1023) | Hygromycin B phosphotransferase ORF | N/C-term 6MYC | x6MYCx | |
| 4436-4693: (258) | T. brucei aldolase mRNA processing 5’ | N-term tdTomato | TOMx | |
| 4680-4913: (234) | EP procyclin promoter | |||
| 4919-5178: (260) | RRNA promoter | Additional selectable markers | ||
| 5182-5194: (13) | Tet operator | BSD | MYCx | NgoMIV – BstZ17I |
| 5198-5292: (95) | T. brucei procyclin mRNA processing 5′ | x6MYCx | ||
| 5293-6747: (1455) | Expression Cassette (GFPx = 1,455 bp) | |||
| 6748-7485: (738) | T. brucei aldolase mRNA processing 3’ | |||
| 7486-7887: (402) | T7 terminator x2 fragment | |||
1 GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT
51 CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT
101 TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT
151 AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA
201 CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA
251 TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG
301 GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT
351 GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA
401 GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG
451 GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC
501 TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA
551 GTCTGGAATT AGAATACATA CCAACTATAT GAGCTCGAAT TCTGCATTAA
601 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
651 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
701 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
751 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
801 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
851 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
901 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
951 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
1001 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
1051 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
1101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
1151 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
1201 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
1251 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
1301 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
1351 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
1401 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1451 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1501 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1551 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1601 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1651 GCCTGACTCC CCGTGGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1701 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1751 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1801 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1851 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1901 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1951 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
2001 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
2051 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
2101 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
2151 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
2201 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
2251 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
2301 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
2351 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
2401 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2451 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2501 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2551 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2601 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2651 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2701 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2751 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2801 CATCAGAGCA GATTGTACTG AGAGTGCACC ATTCGACGCT CTCCCTTATG
2851 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA
2901 CCGCCGCCGC AAGGAATGGT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG
2951 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG
3001 AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC
3051 AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT
3101 AGAGGATCTG GCTAGGAATA CTGCATAGAT AACAAACGCA TCAAACACAA
3151 CTGGTGGCAC TATTTCAATC ATGTCGACAC ACCAAGACGA GAAAATTTGA
3201 GTTAAACAAG ATGCCTACAA CCCTATATTT ATTATCTTCA AGTGATCTTC
3251 TCTTCTCTTG TGCGCTGTAC GTAAATGTGT TGCAAATAAG GCATAGGGCT
3301 GAGTACAGGC ACCACACGCG AGGAAAATCA TAAGCACTAG CCGTGTCAAC
3351 TCACAGCGAC TACAGAACAA TTTTGGCCAC ACAACCCGGT GTTAGGATCT
3401 CCGAGGCCTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC
3451 CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT
3501 CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT
3551 TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA
3601 CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG
3651 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT
3701 CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA
3751 CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT
3801 GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG
3851 CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT
3901 CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC
3951 CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT
4001 AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT
4051 GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC
4101 AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG
4151 TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT
4201 CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA
4251 TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA
4301 TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG
4351 AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC
4401 TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATCACTA GACTTCGTTG
4451 CAGTTGAGTT TCTGTTTTCG GGCTTGTTGG TATACTTGTT TGATTAATGT
4501 GGCTGATAGC TAAGCTATCG CAAATATTTG TACAAGACAT GAGGGGGCAA
4551 AAAAATAAGC CAAGTGTATT TGTTAGGCTT CCGTGCCTGT TAGAGAAATA
4601 TGTAAGGAAA CGCGTGCGCT ACACAGCTTG AGCACCCATC GAATTGGGAC
4651 GAGAACATGT TAAACTCAGT ACTCACCCGA TAACCCCTAT TTTCAATGCA
4701 CGCCCACCCA GAGACACGGA ATAGCATAAA ACAACCCCAT CACTGCCACA
4751 AAATGGACAT TTTGCACAAA ACGCACTATT GACTAGTATT CGACTATACT
4801 AATAAGAGAT ACCTATCAGA CACGATATAC TGTGAAAAGG ACATTAAGCT
4851 CACTATGCAT CTGAAAACGC TTTCACCTCA CAACCTACTT ACACACACCT
4901 TTCCGCTTAA CCCGCTAGCT TTCCACCCAG CGCGGGTGCA TTCTGGCTCT
4951 TATATATACT TATTGTCATG ACAGAGTATA TTGTACTGTG TTGATAAGGG
5001 ACGGGTAACT GTATTGAAGA GCCGATGCTT TTGACATGTT AGATATAATA
5051 TGTTTTATTG TAAAGTCAAT ACAACACACA ATAGGATAAT AATGATAAAG
5101 TTAAAAAAGT ATATATAGTA ATAGAAATAT ATCTTATATA GGAAAGATTA
5151 AGCAGTAAAA GTAGCGCTTA CGGCGTACAC CCTATCAATG ATAGAGTGGT
5201 ACCCTGCACG CGCCTTCGAG TTTTTTTTCC TTTTCCCCAT TTTTTTCAAC
5251 TTGAAGACTT CAATTACACC AAAAAGTAAA ATTCACAAGC TTNNNNNNNN
5301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5801 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5851 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5901 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
5951 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6001 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6051 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
6701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGA
6751 TCCTGCCCAT TTAGTTGGCT TTTCCCTTGT CTCGTGTCTT TTCCGTGGAA
6801 AGGTTCCCGG AGTAATCTGA TGGCACAGCA GGGAGGTGCG CCTGCAGGTT
6851 GGTTAGGAAG GGGGGATGAT GTAAAAGAAG AAAATGGGGG GATATCTTAT
6901 GTTTAAGAGG ATAAATAATG TGAAGGGGCT TTATATGCTT GCTTGGTTGT
6951 CTCGTTGGTG CATGGGGAAT CTGCATGTTT GCTTTGGAGC ACGCGTGGTA
7001 CATCAATGGG ATCATATCTT GCTGCCTCCC GCAGCTCACC GTGCGAGCTG
7051 CCGGGACCCC GTTTCTCATA GGCGTGTGCA CCTTCCGTCT GAGACCTGTA
7101 AATGGTTAAA AAGGAATATA TAATTACCTT TTGAAAAGTG GTAAAAAACG
7151 AATATATCTT TTTTTTTGGT TTTAATACGC TCTTTTGTGT GTATGAGAGG
7201 AATAAATAAG TGTGTGTGTG TGTGTGTGTT GTTGTTGTTG AAAAAATGAA
7251 GGACACGAAA GCGCTCATTA AGGGTGGGGA TACACCAGGC ATAATTATCC
7301 GAAAATATTT TGATGCACCA AATAAGTGAA CATACTGACG AAATCAAAGA
7351 GTTGGAGGAT GGATAGGGAG GCCTCATTGG CAGTGGTAAA TTGATTTGAC
7401 TTGAATATGA TACCCTCTTA CTGTTTCATT GCCACTACAA GTTGGTTTCC
7451 TTCCCCTGCA GGCATGCAAG CTAGGCCACA CCTATCAGCC GGTGTGTTGC
7501 CAAAGACATT CTGTATAATT TTCCAACTAC CCTAACCTGA TTCACTCATC
7551 GTTGAGCATT TGTTCTTCTA CTTTTTGACC GCAAGTGTCG TTATTAGTAT
7601 TCGGTCCTGG GCTGCAGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG
7651 GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA
7701 ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGAACTATAT CCGGATAGGG
7751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG
7801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
7851 TTTGCTGAAA GGAGAACTAT ATCCGGATTG GGCCCCTGCA G