pNATHYG C-terminal in situ tagging construct
Derived from known sequence
| Landmarks | Available expression cassettes | |||
| 0001-1535: (1535) | eGFP Expression cassette | Sequence page | Expression cassette | Region replaced |
| AscI HindIII — 792bp stuffer — XbaI — eGFP — AvrII TGA BamHI | C-terminal 12MYC | x12MYC | XbaI – BamHI | |
| 1536-2166: (631) | T. brucei βα tubulin mRNA processing 5′ | |||
| 2180-3205: (1026) | Hygromycin-B phosphotransferase ORF | |||
| 3209-3498: (290) | T. brucei actin mRNA processing 3′ | Additional selectable markers | ||
| 3499-6034: (2536) | Vector backbone | BSD | xeGFP | KpnI – StuI |
| x12MYC | ||||
1 GGCGCGCCAA GCTTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
51 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
101 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
151 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
251 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
301 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
351 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
601 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
651 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
701 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
751 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
801 NNNNNNTCTA GAGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
851 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
901 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
951 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
1001 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
1051 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
1101 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
1151 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
1201 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
1251 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
1301 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
1351 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
1401 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
1451 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
1501 CTCTCGGCAT GGACGAGCTG TACAAGTGAG GATCCACGCG GACGGGGCAT
1551 TTCCCGTTCG TCATTAGCAG TAGGTAATGA AGATGTTTGT TTCTCGTCCC
1601 CTTTCTCCTT CGTCCTTCTG TCATTTTGTT CTTTTGTGTT TATGTTTTGT
1651 TGTTGTTTTC TTTAATTTTT TTTTTCTTCC ACGTTTGTGT ACATCCGCGC
1701 GCCACTCTAT TCAGAGAGCC ACGGATAGTA GAGGAGGTGG GAAGGGTATA
1751 TGAGGGACAC GCGTACCATG ATGTGGGATG TATTGGGGTC CCTGTCTGTC
1801 CTTACGTGAC TATGTATGAA CCGTCACGTG TAAGATGAGC TAGTGAGATC
1851 AACAGTACAA CTCATCAACA CGCCTTCTTC TCGTTAAATG TACACAATCT
1901 TGATCCTCCA CCTTTATGGG TCCCATTGTT TGCCTCTTCC GCTGTGTGGA
1951 GTGCGCCTAC ACGCACTTCT CACTTCGTAA GTGGTGGTGG CGTAAGTATT
2001 GCCTAATGTT GACTCTATAT TCTCCTCTCC TCACCCCCTC GCGGTGCTGA
2051 TTTCTGACAG ATCTTCAAAC ACTAGTTTAA GCAAAGGACT ATTCATCCGT
2101 TTATATTAGC AACAGTAGGT ACTAGCACCA CTAACAACAA CAACAAAGCA
2151 CTTCTATTTA TTTATCGGTA CCACTAGTGA TGAAAAAGCC TGAACTCACC
2201 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA
2251 CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG
2301 TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC
2351 TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT
2401 TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA
2451 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
2501 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC
2551 GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA
2601 TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC
2651 CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT
2701 CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG
2751 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC
2801 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA
2851 TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
2901 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA
2951 GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT
3001 CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG
3051 GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA
3101 CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT
3151 ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
3201 AATAGAGGCC TCGGAGATCC TAACACCGGG TTGTGTGGCC AAAATTGTTC
3251 TGTAGTCGCT GTGAGTTGAC ACGGCTAGTG CTTATGATTT TCCTCGCGTG
3301 TGGTGCCTGT ACTCAGCCCT ATGCCTTATT TGCAACACAT TTACGTACAG
3351 CGCACAAGAG AAGAGAAGAT CACTTGAAGA TAATAAATAT AGGGTTGTAG
3401 GCATCTTGTT TAACTCAAAT TTTCTCGTCT TGGTGTGTCG ACATGATTGA
3451 AATAGTGCCA CCAGTTGTGT TTGATGCGTT TGTTATCTAT GCAGTATTCC
3501 TAGCCAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA
3551 CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT
3601 CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT
3651 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCACCATT
3701 CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC
3751 TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAATGGTGCA CTCTCAGTAC
3801 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
3851 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA
3901 CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
3951 ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT
4001 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT
4051 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
4101 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
4151 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
4201 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
4251 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
4301 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA
4351 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT
4401 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
4451 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
4501 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
4551 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
4601 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT
4651 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC
4701 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
4751 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
4801 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
4851 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
4901 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG
4951 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT
5001 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT
5051 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
5101 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
5151 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
5201 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
5251 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
5301 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC
5351 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
5401 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
5451 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
5501 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
5551 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
5601 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
5651 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
5701 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
5751 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
5801 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
5851 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
5901 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG
5951 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
6001 CGTTGGCCGA TTCATTAATG CAGAATTCGA GCTC