pNATBSD N-terminal in situ tagging construct
pNATBSD N-terminal in situ tagging construct
Derived from known sequence
| Landmarks | Available expression cassettes | |||
| 0001-2497: (2497) | Vector backbone | Sequence page | Expression cassette | Region replaced |
| 2498-2736: (239) | T. brucei βα tubulin mRNA processing 5′ | N-terminal eGFP | GFPx | HindIII – BamHI |
| 2747-3145: (399) | Blasticidin-S deaminase ORF | |||
| 3161-3483: (323) | T. brucei αβ tubulin mRNA processing 3′ | Additional selectable markers | ||
| 3484-3728: (245) | 6MYC Expression Cassette | HYG | 6MYCx | SpeI – SacI |
| HindIII PacI ATG XbaI — [cMYC]6 — AvrII TGA BamHI | GFPx | |||
1 GAATTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
51 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
101 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
151 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
201 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
251 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
301 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
351 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
451 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
501 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
551 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
601 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
651 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
751 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
801 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
851 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
901 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
951 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
1001 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
1051 GTTCATCCAT AGTTGCCTGA CTCCCCGTGG TGTAGATAAC TACGATACGG
1101 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
1151 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
1201 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
1251 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
1301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
1351 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
1401 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
1451 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
1501 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
1551 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
1601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
1651 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
1701 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
1751 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
1801 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
1851 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
1901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
1951 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA
2001 GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
2051 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA
2101 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
2151 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG
2201 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATTCGA
2251 CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT
2301 GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCAAGG AGATGGCGCC
2351 CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG
2401 CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG
2451 GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCTGG
2501 GTCCCATTGT TTGCCTCTTC CGCTGTGTGG AGTGCGCCTA CACGCACTTC
2551 TCACTTCGTA AGTGGTGGTG GCGTAAGTAT TGCCTAATGT TGACTCTATG
2601 TTCTCCTCTC CTCACCCCCT CGCGGTGCTG ATTTCTGACA GATCTTCAAA
2651 CACTAGTTTA AGCAAAGGAC TATTCATCCG TTTATATTAG CAACAGTAGG
2701 TACTAGCACC ACTAACAACA ACAACAAAGC ACTTCTGCAG GAATTCATGG
2751 CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT
2801 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC
2851 TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA
2901 CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
2951 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG
3001 CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC
3051 CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT
3101 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAGAATT
3151 CGAGCTCGCC CTAGAAAGTG TGACAACGTC GCACCATGTG TAGGTTTTCA
3201 TTTATGTTCT TTCTTTCTTT CTTTTGTGAA TTTGTTTTCT GTCTCAAATG
3251 TTTTTAATTC GCTTGGGACC TATGTTTTTC TTGTTTTTTT GCTCACCCTT
3301 TGTGTAGGAG GCACCCTGTC ACGTCTGTGG TTGCGTGTAT GCCTTCCTTC
3351 CCCTTATTCG CTTCTTCCTG TCGTGTCACA CCTCTTTCTC CCTCTCCCTT
3401 TCCGCCTTTT CTTTCAATCT TGTTTTCTCG ACCAGCCCTA CTAGAGGAGA
3451 AAGAATAGTA ACCCTTTCAT CAAAGAAAAT AGTAAGCTTA ATTAATGTCT
3501 AGAGAACAGA AACTGATCTC TGAAGAAGAC CTGCCTAGAG AACAGAAACT
3551 GATCTCTGAA GAAGACCTGC CTAGAGAACA GAAACTGATC TCTGAAGAAG
3601 ACCTGCCTAG AGAACAGAAA CTGATCTCTG AAGAAGACCT GCCTAGAGAA
3651 CAGAAACTGA TCTCTGAAGA AGACCTGCCT AGAGAACAGA AACTGATCTC
3701 TGAAGAAGAC CTGCCTAGGT GAGGATCC