p2T7TAblue sequence
in silico assembly, October 2005
| Landmarks | ||||
| 0001-0592: (592) | RRNA spacer targeting fragment Not “I” | |||
| 0593-3116: (2524) | Vector backbone (pUC/pGEM) | |||
| 3117-3412: (296) | T. brucei actin mRNA processing 3’ | |||
| 3413-4435: (1023) | Hygromycin B phosphotransferase ORF | |||
| 4436-4570: (135) | T. brucei actin mRNA processing 5’ | |||
| 4571-4865: (295) | RRNA promoter | |||
| 4866-5338: (473) | T7 terminator x2 fragment | |||
| 5339-5358: (20) | T7 promoter | |||
| 5359-5418: (60) | Tet operator fragment | |||
| 5419-6246: (828) | LacZ ‘stuffer’ | |||
| 6247-6333: (87) | Tet operator fragment | |||
| 6334-6353: (20) | T7 promoter | |||
| 6354-6755: (402) | T7 terminator x2 fragment | |||
1 GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT
51 CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT
101 TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT
151 AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA
201 CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA
251 TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG
301 GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT
351 GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA
401 GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG
451 GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC
501 TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA
551 GTCTGGAATT AGAATACATA CCAACTATAT GAGCTCGAAT TCTGCATTAA
601 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
651 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
701 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
751 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
801 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
851 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
901 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
951 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
1001 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
1051 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
1101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
1151 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
1201 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
1251 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
1301 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
1351 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
1401 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1451 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1501 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1551 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1601 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1651 GCCTGACTCC CCGTGGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1701 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1751 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1801 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1851 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1901 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1951 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
2001 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
2051 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
2101 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
2151 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
2201 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
2251 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
2301 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
2351 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
2401 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2451 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2501 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2551 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2601 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2651 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2701 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2751 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2801 CATCAGAGCA GATTGTACTG AGAGTGCACC ATTCGACGCT CTCCCTTATG
2851 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA
2901 CCGCCGCCGC AAGGAATGGT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG
2951 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG
3001 AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC
3051 AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT
3101 AGAGGATCTG GCTAGGAATA CTGCATAGAT AACAAACGCA TCAAACACAA
3151 CTGGTGGCAC TATTTCAATC ATGTCGACAC ACCAAGACGA GAAAATTTGA
3201 GTTAAACAAG ATGCCTACAA CCCTATATTT ATTATCTTCA AGTGATCTTC
3251 TCTTCTCTTG TGCGCTGTAC GTAAATGTGT TGCAAATAAG GCATAGGGCT
3301 GAGTACAGGC ACCACACGCG AGGAAAATCA TAAGCACTAG CCGTGTCAAC
3351 TCACAGCGAC TACAGAACAA TTTTGGCCAC ACAACCCGGT GTTAGGATCT
3401 CCGAGGCCTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC
3451 CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT
3501 CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT
3551 TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA
3601 CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG
3651 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT
3701 CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA
3751 CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT
3801 GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG
3851 CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT
3901 CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC
3951 CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT
4001 AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT
4051 GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC
4101 AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG
4151 TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT
4201 CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA
4251 TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA
4301 TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG
4351 AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC
4401 TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATCACTA GTTCTAGAGC
4451 TTATTTTATG GCAGCAACGA GACCTTACGT AAAGCAAAAC TAAATAAGAG
4501 CGGAGACTGC AATGCAGAGT AAAAAAAAAA AAAACATGAA TTTCAGAAGA
4551 CCTTGCTGTG CCCGGGCTCT GAGAGCGGTC AGTTGCTCTC CTGCTCCGTA
4601 CGCCGTAAGC GCTACTTTTA CTGCTTAATC TTTCCTATAT AAGATATATT
4651 TCTATTACTA TATATACTTT TTTAACTTTA TCATTATTAT CCTATTGTGT
4701 GTTGTATTGA CTTTACAATA AAACATATTA TATCTAACAT GTCAAAAGCA
4751 TCGGCTCTTC AATACAGTTA CCCGTCCCTT ATCAACACAG TACAATATAC
4801 TCTGTCATGA CAATAAGTAT ATATAAGAGC CAGAATGCAC CCGCGCTGGG
4851 TGGAAAGCTA GCTTGCATGC CTGCAGGGGC CCAATCCGGA TATAGTTCTC
4901 CTTTCAGCAA AAAACCCCTC AAGACCCGTT TAGAGGCCCC AAGGGGTTAT
4951 GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT CCTTTCGGGC
5001 TTTGTTAGCA GCCCTATCCG GATATAGTTC TCCTTTCAGC AAAAAACCCC
5051 TCAAGACCCG TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG
5101 GTGGCAGCAG CCAACTCAGC TTCCTTTCGG GCTTTGTTAG CAGCCTGCAG
5151 AATACTGCAT AGATAACAAA CGCATCAAAC ACAACTGGTG GCACTATTTC
5201 AATCATGTCG AGGAGGACCG AATACTAATA ACGACACTTG CGGTCAAAAA
5251 GTAGAAGAAC AAATGCTCAA CGATGAGTGA ATCAGGTTAG GGTAGTCGGA
5301 AAATTATACA GAATGTCTTT GGCAACACAC CGGCTGATTA ATACGACTCA
5351 CTATAGGGAG ATCTCCCTAT CAGTGATAGA GATCTCCCCG GGCCCCCCCT
5401 CGAGTTAACG ACCTTGCGTC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG
5451 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA
5501 AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA
5551 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
5601 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA
5651 AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA
5701 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT
5751 ACAGGGCGCG TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
5801 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
5851 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT
5901 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT
5951 GGGCCCGACG TCGCATGCTC CCGGCCGCCA TGGCGGCCGC GGGAATTCGA
6001 TATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACCATA TGGGAGAGCT
6051 CCCAACGCGT TGGATGCATA GCTTGAGTAT TCTATAGTGT CACCTAAATA
6101 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
6151 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
6201 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCGACGCAA
6251 GGTCGTTAAC GGATCCACTA GTTCTAGAGC GGCCGGCCGC CACCGCGGCT
6301 AGATCTCTAT CACTGATAGG GAGATCTCCC TATAGTGAGT CGTATTAATC
6351 AGCCGGTGTG TTGCCAAAGA CATTCTGTAT AATTTTCCAA CTACCCTAAC
6401 CTGATTCACT CATCGTTGAG CATTTGTTCT TCTACTTTTT GACCGCAAGT
6451 GTCGTTATTA GTATTCGGTC CTGGGCTGCA GGCTGCTAAC AAAGCCCGAA
6501 AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC
6551 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGAACT
6601 ATATCCGGAT AGGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG
6651 CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
6701 GTCTTGAGGG GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATTGGGCCCC
6751 TGCAG