p2T7aTAblue sequence
Derived from known sequence and junction sequencing, February 2008
Landmarks
| 0001-0600: (600) | RRNA targeting fragment AscI |
| 0601-3124: (2525) | Vector backbone (pUC/pGEM) |
| 3125-3186: (62) | Remnant of T. brucei actin mRNA processing 3’ |
| 3187-4207: (1021) | Hygromycin B phosphotransferase ORF ΔSTOP AscI |
| 4208-4339: (132) | T. brucei actin mRNA processing 5’ |
| 4340-4629: (290) | RRNA promoter |
| 4638-5110: (473) | T7 terminator x2 fragment |
| 5111-5130: (20) | T7 promoter |
| 5139-5151: (13) | Tet operator |
| 5188-6021: (828) | LacZ ‘stuffer’ |
| 6079-6091: (13) | Tet operator |
| 6100-6119: (20) | T7 promoter |
| 6122-6523: (402) | T7 terminator x2 fragment |
1 GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT
51 CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT
101 TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT
151 AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA
201 CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA
251 TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG
301 GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT
351 GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA
401 GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG
451 GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC
501 TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA
551 GTCTGGAATT AGAATACATA CCAACTATAT GGCGCGCCGA GCTCGAATTC
601 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
651 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
751 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
801 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
851 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
901 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
951 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
1001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
1051 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
1101 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
1151 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
1201 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
1251 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
1301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
1351 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
1401 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
1451 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
1501 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
1551 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
1601 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
1651 CCATAGTTGC CTGACTCCCC GTGGTGTAGA TAACTACGAT ACGGGAGGGC
1701 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
1751 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
1801 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
1851 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
1901 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
1951 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
2001 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
2051 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
2101 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
2151 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
2201 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
2251 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
2301 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
2351 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
2401 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
2451 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
2501 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
2551 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC
2601 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
2651 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
2701 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
2751 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
2801 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT TCGACGCTCT
2851 CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC
2901 GTTGAGCACC GCCGCCGCAA GGAATGGTGC AAGGAGATGG CGCCCAACAG
2951 TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA
3001 TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA
3051 TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG
3101 TCCGGCGTAG AGGATCTGGC TAGGAATACT GCATAGATAA CAAACGCATC
3151 AAACACAACT GGTGGCACTA TTTCAATCAT GTCGACGGCG CGCCCTCGGA
3201 CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC
3251 ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG
3301 TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT
3351 GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC
3401 AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT
3451 GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC
3501 CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC
3551 CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT
3601 TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC
3651 TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC
3701 GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG
3751 CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG
3801 AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
3851 CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA
3901 GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG
3951 CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC
4001 CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC
4051 AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA
4101 GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC
4151 GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT
4201 TTTTCATCAC TAGTTCTAGA GCTTATTTTA TGGCAGCAAC GAGACCTTAC
4251 GTAAAGCAAA ACTAAATAAG AGCGGAGACT GCAATGCAGA GTAAAAAAAA
4301 AAAAAACATG AATTTCAGAA GACCTTGCTG TGCCCGGGCT CTGAGAGCGG
4351 TCAGTTGCTC TCCTGCTCCG TACGCCGTAA GCGCTACTTT TACTGCTTAA
4401 TCTTTCCTAT ATAAGATATA TTTCTATTAC TATATATACT TTTTTAACTT
4451 TATCATTATT ATCCTATTGT GTGTTGTATT GACTTTACAA TAAAACATAT
4501 TATATCTAAC ATGTCAAAAG CATCGGCTCT TCAATACAGT TACCCGTCCC
4551 TTATCAACAC AGTACAATAT ACTCTGTCAT GACAATAAGT ATATATAAGA
4601 GCCAGAATGC ACCCGCGCTG GGTGGAAAGC TAGCTTGCAT GCCTGCAGGG
4651 GCCCAATCCG GATATAGTTC TCCTTTCAGC AAAAAACCCC TCAAGACCCG
4701 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG
4751 CCAACTCAGC TTCCTTTCGG GCTTTGTTAG CAGCCCTATC CGGATATAGT
4801 TCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG CCCCAAGGGG
4851 TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
4901 GGGCTTTGTT AGCAGCCTGC AGAATACTGC ATAGATAACA AACGCATCAA
4951 ACACAACTGG TGGCACTATT TCAATCATGT CGAGGAGGAC CGAATACTAA
5001 TAACGACACT TGCGGTCAAA AAGTAGAAGA ACAAATGCTC AACGATGAGT
5051 GAATCAGGTT AGGGTAGTCG GAAAATTATA CAGAATGTCT TTGGCAACAC
5101 ACCGGCTGAT TAATACGACT CACTATAGGG AGATCTCCCT ATCAGTGATA
5151 GAGATCTCCC CGGGCCCCCC CTCGAGTTAA CGACCTTGCG TCCGAGATAG
5201 GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG
5251 GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT
5301 ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG
5351 CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA
5401 AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
5451 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
5501 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCATTCG CCATTCAGGC
5551 TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
5601 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC
5651 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTGTAAT
5701 ACGACTCACT ATAGGGCGAA TTGGGCCCGA CGTCGCATGC TCCCGGCCGC
5751 CATGGCGGCC GCGGGAATTC GATATCACTA GTGAATTCGC GGCCGCCTGC
5801 AGGTCGACCA TATGGGAGAG CTCCCAACGC GTTGGATGCA TAGCTTGAGT
5851 ATTCTATAGT GTCACCTAAA TAGCTTGGCG TAATCATGGT CATAGCTGTT
5901 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG
5951 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
6001 TTAATTGCGT TGCGCGACGC AAGGTCGTTA ACGGATCCAC TAGTTCTAGA
6051 GCGGCCGGCC GCCACCGCGG CTAGATCTCT ATCACTGATA GGGAGATCTC
6101 CCTATAGTGA GTCGTATTAA TCAGCCGGTG TGTTGCCAAA GACATTCTGT
6151 ATAATTTTCC AACTACCCTA ACCTGATTCA CTCATCGTTG AGCATTTGTT
6201 CTTCTACTTT TTGACCGCAA GTGTCGTTAT TAGTATTCGG TCCTGGGCTG
6251 CAGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC
6301 TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG
6351 GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATAGGGCTGC TAACAAAGCC
6401 CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA
6451 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG
6501 AACTATATCC GGATTGGGCC CCTGCAG