Derived from known sequence

SpeI — HYG — SacI

    1  ACTAGTGATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
   51  TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC
  101  GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT
  151  GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC
  201  GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG
  251  GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT
  301  CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
  351  TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC
  401  GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG
  451  TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG
  501  TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG
  551  ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA
  601  TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA
  651  TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC
  701  ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA
  751  CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT
  801  ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC
  851  AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG
  901  ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG
  951  CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA
 1001  CGCCCCAGCA CTCGTCCGAG GGCAAAGGAA TAGAGCTC