{"id":359,"date":"2023-10-16T11:50:55","date_gmt":"2023-10-16T10:50:55","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=359"},"modified":"2026-02-13T15:54:03","modified_gmt":"2026-02-13T15:54:03","slug":"n-term-egfp","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/n-term-egfp\/","title":{"rendered":"N-term eGFP"},"content":{"rendered":"\n<p>Cassette sequenced in full<\/p>\n\n\n\n<pre class=\"wp-block-preformatted\"><strong><sup>GFP<\/sup>x<\/strong>\n\n<em>Hin<\/em>dIII -- START <em>eGFP<\/em> -- <em>Xba<\/em>I -- 732 or 1812 bp stuffer -- <em>Bam<\/em>HI\n    1  <em>AAGCTT<\/em><strong>ATG<\/strong>G TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT\n   51  CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG\n  101  GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC\n  151  TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT\n  201  GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC\n  251  ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC\n  301  ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT\n  351  CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA\n  401  AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC\n  451  CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA\n  501  CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC\n  551  ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC\n  601  AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA\n  651  GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC\n  701  TCGGCATGGA CGAGCTGTAC AAGTCTAGAN NNNNNNNNNN NNNNNNNNNN\n  751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 1951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 2501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGATC C<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Cassette sequenced in full GFPx HindIII &#8212; START eGFP &#8212; XbaI &#8212; 732 or 1812 bp stuffer &#8212; BamHI 1 AAGCTTATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT 51 CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 101 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC 151 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 201 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC 251 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-359","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/359","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=359"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/359\/revisions"}],"predecessor-version":[{"id":574,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/359\/revisions\/574"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=359"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}