{"id":345,"date":"2023-10-16T11:03:20","date_gmt":"2023-10-16T10:03:20","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=345"},"modified":"2026-02-13T15:53:38","modified_gmt":"2026-02-13T15:53:38","slug":"inducible-expression-data-bloodstream-form-t-brucei","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/inducible-expression-data-bloodstream-form-t-brucei\/","title":{"rendered":"Inducible expression data (bloodstream form T. brucei)"},"content":{"rendered":"\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<p><strong>Time Course (hrs)<\/strong><br>\u2013 cells were incubated with 1\u03bcg ml-1 tetracycline<\/p>\n\n\n\n<figure class=\"wp-block-image alignfull size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"334\" height=\"87\" src=\"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-content\/uploads\/sites\/232\/2023\/10\/pTime_2.jpg\" alt=\"image of cells\" class=\"wp-image-348\" srcset=\"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-content\/uploads\/sites\/232\/2023\/10\/pTime_2.jpg 334w, https:\/\/sites.dundee.ac.uk\/david-horn\/wp-content\/uploads\/sites\/232\/2023\/10\/pTime_2-300x78.jpg 300w\" sizes=\"auto, (max-width: 334px) 100vw, 334px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<p><strong>Dose Response<\/strong><br>\u2013 ng ml-1 tetracycline, 24h<\/p>\n\n\n\n<figure class=\"wp-block-image alignfull size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"290\" height=\"80\" src=\"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-content\/uploads\/sites\/232\/2023\/10\/pDose_1.jpg\" alt=\"image of cells\" class=\"wp-image-347\"\/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p>Whole cell lysates from cultured Single Marker bloodstream form\u00a0<em>T. brucei<\/em>\u00a0expressing a cMyc-tagged protein (pT7TAG integrated at a\u00a0<em>RRNA<\/em>\u00a0spacer locus) were frationated by SDS-PAGE, transferred to nitrocellulose membrane and incubated with anti-cMyc (4A6, Upstate) and detected using ECL+ (Amersham).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Time Course (hrs)\u2013 cells were incubated with 1\u03bcg ml-1 tetracycline Dose Response\u2013 ng ml-1 tetracycline, 24h Whole cell lysates from cultured Single Marker bloodstream form\u00a0T. brucei\u00a0expressing a cMyc-tagged protein (pT7TAG integrated at a\u00a0RRNA\u00a0spacer locus) were frationated by SDS-PAGE, transferred to nitrocellulose membrane and incubated with anti-cMyc (4A6, Upstate) and detected using ECL+ (Amersham).<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-345","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/345","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=345"}],"version-history":[{"count":5,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/345\/revisions"}],"predecessor-version":[{"id":573,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/345\/revisions\/573"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=345"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}