{"id":340,"date":"2023-10-16T10:55:14","date_gmt":"2023-10-16T09:55:14","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=340"},"modified":"2026-02-13T15:56:38","modified_gmt":"2026-02-13T15:56:38","slug":"p2t7atablue-sequence","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/p2t7atablue-sequence\/","title":{"rendered":"p2T7aTAblue<\/sup> sequence"},"content":{"rendered":"\n

Derived from known sequence and junction sequencing, February 2008<\/p>\n\n\n\n

Landmarks<\/strong><\/p>\n\n\n\n

0001-0600: (600)<\/td>RRNA<\/em> targeting fragment AscI<\/td><\/tr>
0601-3124: (2525)<\/td>Vector backbone (pUC\/pGEM)<\/td><\/tr>
3125-3186: (62)<\/td>Remnant of T. brucei<\/em> actin mRNA processing 3\u2019<\/td><\/tr>
3187-4207: (1021)<\/td>Hygromycin B phosphotransferase ORF \u0394STOP AscI<\/td><\/tr>
4208-4339: (132)<\/td>T. brucei<\/em> actin mRNA processing 5\u2019<\/td><\/tr>
4340-4629: (290)<\/td>RRNA promoter<\/td><\/tr>
4638-5110: (473)<\/td>T7 terminator x2 fragment<\/td><\/tr>
5111-5130: (20)<\/td>T7 promoter<\/td><\/tr>
5139-5151: (13)<\/td>Tet operator<\/td><\/tr>
5188-6021: (828)<\/td>LacZ<\/em> \u2018stuffer\u2019<\/td><\/tr>
6079-6091: (13)<\/td>Tet operator<\/td><\/tr>
6100-6119: (20)<\/td>T7 promoter<\/td><\/tr>
6122-6523: (402)<\/td>T7 terminator x2 fragment<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    1  GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT\n   51  CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT\n  101  TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT\n  151  AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA\n  201  CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA\n  251  TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG\n  301  GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT\n  351  GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA\n  401  GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG\n  451  GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC\n  501  TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA\n  551  GTCTGGAATT AGAATACATA CCAACTATAT GGCGCGCCGA GCTCGAATTC\n  601  TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG\n  651  CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT\n  701  GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG\n  751  AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG\n  801  GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG\n  851  CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA\n  901  ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC\n  951  GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT\n 1001  TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC\n 1051  TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC\n 1101  CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC\n 1151  CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA\n 1201  GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG\n 1251  TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT\n 1301  GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA\n 1351  AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT\n 1401  ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG\n 1451  GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA\n 1501  GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT\n 1551  TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA\n 1601  ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT\n 1651  CCATAGTTGC CTGACTCCCC GTGGTGTAGA TAACTACGAT ACGGGAGGGC\n 1701  TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC\n 1751  GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA\n 1801  GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC\n 1851  CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT\n 1901  TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT\n 1951  CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG\n 2001  TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG\n 2051  TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT\n 2101  CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC\n 2151  TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG\n 2201  CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG\n 2251  TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA\n 2301  CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC\n 2351  TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA\n 2401  GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA\n 2451  CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG\n 2501  TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG\n 2551  GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC\n 2601  ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT\n 2651  TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC\n 2701  TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA\n 2751  GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA\n 2801  CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT TCGACGCTCT\n 2851  CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC\n 2901  GTTGAGCACC GCCGCCGCAA GGAATGGTGC AAGGAGATGG CGCCCAACAG\n 2951  TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA\n 3001  TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA\n 3051  TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG\n 3101  TCCGGCGTAG AGGATCTGGC TAGGAATACT GCATAGATAA CAAACGCATC\n 3151  AAACACAACT GGTGGCACTA TTTCAATCAT GTCGACGGCG CGCCCTCGGA\n 3201  CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC\n 3251  ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG\n 3301  TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT\n 3351  GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC\n 3401  AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT\n 3451  GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC\n 3501  CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC\n 3551  CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT\n 3601  TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC\n 3651  TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC\n 3701  GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG\n 3751  CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG\n 3801  AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC\n 3851  CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA\n 3901  GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG\n 3951  CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC\n 4001  CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC\n 4051  AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA\n 4101  GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC\n 4151  GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT\n 4201  TTTTCATCAC TAGTTCTAGA GCTTATTTTA TGGCAGCAAC GAGACCTTAC\n 4251  GTAAAGCAAA ACTAAATAAG AGCGGAGACT GCAATGCAGA GTAAAAAAAA\n 4301  AAAAAACATG AATTTCAGAA GACCTTGCTG TGCCCGGGCT CTGAGAGCGG\n 4351  TCAGTTGCTC TCCTGCTCCG TACGCCGTAA GCGCTACTTT TACTGCTTAA\n 4401  TCTTTCCTAT ATAAGATATA TTTCTATTAC TATATATACT TTTTTAACTT\n 4451  TATCATTATT ATCCTATTGT GTGTTGTATT GACTTTACAA TAAAACATAT\n 4501  TATATCTAAC ATGTCAAAAG CATCGGCTCT TCAATACAGT TACCCGTCCC\n 4551  TTATCAACAC AGTACAATAT ACTCTGTCAT GACAATAAGT ATATATAAGA\n 4601  GCCAGAATGC ACCCGCGCTG GGTGGAAAGC TAGCTTGCAT GCCTGCAGGG\n 4651  GCCCAATCCG GATATAGTTC TCCTTTCAGC AAAAAACCCC TCAAGACCCG\n 4701  TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG\n 4751  CCAACTCAGC TTCCTTTCGG GCTTTGTTAG CAGCCCTATC CGGATATAGT\n 4801  TCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG CCCCAAGGGG\n 4851  TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC\n 4901  GGGCTTTGTT AGCAGCCTGC AGAATACTGC ATAGATAACA AACGCATCAA\n 4951  ACACAACTGG TGGCACTATT TCAATCATGT CGAGGAGGAC CGAATACTAA\n 5001  TAACGACACT TGCGGTCAAA AAGTAGAAGA ACAAATGCTC AACGATGAGT\n 5051  GAATCAGGTT AGGGTAGTCG GAAAATTATA CAGAATGTCT TTGGCAACAC\n 5101  ACCGGCTGAT TAATACGACT CACTATAGGG AGATCTCCCT ATCAGTGATA\n 5151  GAGATCTCCC CGGGCCCCCC CTCGAGTTAA CGACCTTGCG TCCGAGATAG\n 5201  GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG\n 5251  GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT\n 5301  ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG\n 5351  CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA\n 5401  AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG\n 5451  CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC\n 5501  CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCATTCG CCATTCAGGC\n 5551  TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC\n 5601  CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC\n 5651  AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTGTAAT\n 5701  ACGACTCACT ATAGGGCGAA TTGGGCCCGA CGTCGCATGC TCCCGGCCGC\n 5751  CATGGCGGCC GCGGGAATTC GATATCACTA GTGAATTCGC GGCCGCCTGC\n 5801  AGGTCGACCA TATGGGAGAG CTCCCAACGC GTTGGATGCA TAGCTTGAGT\n 5851  ATTCTATAGT GTCACCTAAA TAGCTTGGCG TAATCATGGT CATAGCTGTT\n 5901  TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG\n 5951  GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA\n 6001  TTAATTGCGT TGCGCGACGC AAGGTCGTTA ACGGATCCAC TAGTTCTAGA\n 6051  GCGGCCGGCC GCCACCGCGG CTAGATCTCT ATCACTGATA GGGAGATCTC\n 6101  CCTATAGTGA GTCGTATTAA TCAGCCGGTG TGTTGCCAAA GACATTCTGT\n 6151  ATAATTTTCC AACTACCCTA ACCTGATTCA CTCATCGTTG AGCATTTGTT\n 6201  CTTCTACTTT TTGACCGCAA GTGTCGTTAT TAGTATTCGG TCCTGGGCTG\n 6251  CAGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC\n 6301  TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG\n 6351  GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATAGGGCTGC TAACAAAGCC\n 6401  CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA\n 6451  ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG\n 6501  AACTATATCC GGATTGGGCC CCTGCAG<\/pre>\n","protected":false},"excerpt":{"rendered":"
Derived from known sequence and junction sequencing, February 2008 Landmarks 0001-0600: (600) RRNA targeting fragment AscI 0601-3124: (2525) Vector backbone (pUC\/pGEM) 3125-3186: (62) Remnant of T. brucei actin mRNA processing 3\u2019 3187-4207: (1021) Hygromycin B phosphotransferase ORF \u0394STOP AscI 4208-4339: (132) T. brucei actin mRNA processing 5\u2019 4340-4629: (290) RRNA promoter 4638-5110: (473) T7 terminator x2 fragment 5111-5130: (20) […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-340","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/340","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=340"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/340\/revisions"}],"predecessor-version":[{"id":580,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/340\/revisions\/580"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}