{"id":336,"date":"2023-10-16T10:51:33","date_gmt":"2023-10-16T09:51:33","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=336"},"modified":"2026-02-13T15:56:07","modified_gmt":"2026-02-13T15:56:07","slug":"p2t7tablue-sequence","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/p2t7tablue-sequence\/","title":{"rendered":"p2T7TAblue<\/sup> sequence"},"content":{"rendered":"\n

in silico<\/em> assembly, October 2005<\/p>\n\n\n\n

Landmarks<\/strong><\/td><\/td><\/tr>
0001-0592: (592)<\/td>RRNA<\/em> spacer targeting fragment Not<\/em> \u201cI\u201d<\/td><\/td><\/td><\/td><\/tr>
0593-3116: (2524)<\/td>Vector backbone (pUC\/pGEM)<\/td><\/td><\/td><\/td><\/tr>
3117-3412: (296)<\/td>T. brucei<\/em> actin mRNA processing 3\u2019<\/td><\/td><\/td><\/td><\/tr>
3413-4435: (1023)<\/td>Hygromycin B phosphotransferase ORF<\/td><\/td><\/td><\/td><\/tr>
4436-4570: (135)<\/td>T. brucei<\/em> actin mRNA processing 5\u2019<\/td><\/td><\/td><\/td><\/tr>
4571-4865: (295)<\/td>RRNA promoter<\/td><\/td><\/td><\/td><\/tr>
4866-5338: (473)<\/td>T7 terminator x2 fragment<\/td><\/td><\/td><\/td><\/tr>
5339-5358: (20)<\/td>T7 promoter<\/td><\/td><\/td><\/td><\/tr>
5359-5418: (60)<\/td>Tet operator fragment<\/td><\/td><\/td><\/td><\/tr>
5419-6246: (828)<\/td>LacZ<\/em> \u2018stuffer\u2019<\/td><\/td><\/td><\/td><\/tr>
6247-6333: (87)<\/td>Tet operator fragment<\/td><\/td><\/td><\/td><\/tr>
6334-6353: (20)<\/td>T7 promoter<\/td><\/td><\/td><\/td><\/tr>
6354-6755: (402)<\/td>T7 terminator x2 fragment<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
          1  GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT\n      51  CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT\n     101  TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT\n     151  AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA\n     201  CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA\n     251  TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG\n     301  GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT\n     351  GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA\n     401  GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG\n     451  GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC\n     501  TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA\n     551  GTCTGGAATT AGAATACATA CCAACTATAT GAGCTCGAAT TCTGCATTAA\n     601  TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC\n     651  CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG\n     701  CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG\n     751  GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA\n     801  CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG\n     851  ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA\n     901  GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC\n     951  TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT\n    1001  CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG\n    1051  GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA\n    1101  GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG\n    1151  TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC\n    1201  AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA\n    1251  CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC\n    1301  CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC\n    1351  ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG\n    1401  AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG\n    1451  CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA\n    1501  AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC\n    1551  AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA\n    1601  TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT\n    1651  GCCTGACTCC CCGTGGTGTA GATAACTACG ATACGGGAGG GCTTACCATC\n    1701  TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG\n    1751  ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT\n    1801  CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC\n    1851  TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG\n    1901  CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC\n    1951  TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA\n    2001  AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG\n    2051  CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT\n    2101  GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA\n    2151  GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT\n    2201  CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC\n    2251  ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT\n    2301  GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT\n    2351  CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT\n    2401  GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT\n    2451  CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA\n    2501  GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG\n    2551  CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT\n    2601  CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG\n    2651  CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG\n    2701  ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA\n    2751  GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG\n    2801  CATCAGAGCA GATTGTACTG AGAGTGCACC ATTCGACGCT CTCCCTTATG\n    2851  CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA\n    2901  CCGCCGCCGC AAGGAATGGT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG\n    2951  CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG\n    3001  AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC\n    3051  AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT\n    3101  AGAGGATCTG GCTAGGAATA CTGCATAGAT AACAAACGCA TCAAACACAA\n    3151  CTGGTGGCAC TATTTCAATC ATGTCGACAC ACCAAGACGA GAAAATTTGA\n    3201  GTTAAACAAG ATGCCTACAA CCCTATATTT ATTATCTTCA AGTGATCTTC\n    3251  TCTTCTCTTG TGCGCTGTAC GTAAATGTGT TGCAAATAAG GCATAGGGCT\n    3301  GAGTACAGGC ACCACACGCG AGGAAAATCA TAAGCACTAG CCGTGTCAAC\n    3351  TCACAGCGAC TACAGAACAA TTTTGGCCAC ACAACCCGGT GTTAGGATCT\n    3401  CCGAGGCCTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC\n    3451  CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT\n    3501  CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT\n    3551  TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA\n    3601  CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG\n    3651  AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT\n    3701  CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA\n    3751  CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT\n    3801  GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG\n    3851  CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT\n    3901  CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC\n    3951  CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT\n    4001  AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT\n    4051  GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC\n    4101  AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG\n    4151  TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT\n    4201  CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA\n    4251  TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA\n    4301  TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG\n    4351  AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC\n    4401  TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATCACTA GTTCTAGAGC\n    4451  TTATTTTATG GCAGCAACGA GACCTTACGT AAAGCAAAAC TAAATAAGAG\n    4501  CGGAGACTGC AATGCAGAGT AAAAAAAAAA AAAACATGAA TTTCAGAAGA\n    4551  CCTTGCTGTG CCCGGGCTCT GAGAGCGGTC AGTTGCTCTC CTGCTCCGTA\n    4601  CGCCGTAAGC GCTACTTTTA CTGCTTAATC TTTCCTATAT AAGATATATT\n    4651  TCTATTACTA TATATACTTT TTTAACTTTA TCATTATTAT CCTATTGTGT\n    4701  GTTGTATTGA CTTTACAATA AAACATATTA TATCTAACAT GTCAAAAGCA\n    4751  TCGGCTCTTC AATACAGTTA CCCGTCCCTT ATCAACACAG TACAATATAC\n    4801  TCTGTCATGA CAATAAGTAT ATATAAGAGC CAGAATGCAC CCGCGCTGGG\n    4851  TGGAAAGCTA GCTTGCATGC CTGCAGGGGC CCAATCCGGA TATAGTTCTC\n    4901  CTTTCAGCAA AAAACCCCTC AAGACCCGTT TAGAGGCCCC AAGGGGTTAT\n    4951  GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT CCTTTCGGGC\n    5001  TTTGTTAGCA GCCCTATCCG GATATAGTTC TCCTTTCAGC AAAAAACCCC\n    5051  TCAAGACCCG TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG\n    5101  GTGGCAGCAG CCAACTCAGC TTCCTTTCGG GCTTTGTTAG CAGCCTGCAG\n    5151  AATACTGCAT AGATAACAAA CGCATCAAAC ACAACTGGTG GCACTATTTC\n    5201  AATCATGTCG AGGAGGACCG AATACTAATA ACGACACTTG CGGTCAAAAA\n    5251  GTAGAAGAAC AAATGCTCAA CGATGAGTGA ATCAGGTTAG GGTAGTCGGA\n    5301  AAATTATACA GAATGTCTTT GGCAACACAC CGGCTGATTA ATACGACTCA\n    5351  CTATAGGGAG ATCTCCCTAT CAGTGATAGA GATCTCCCCG GGCCCCCCCT\n    5401  CGAGTTAACG ACCTTGCGTC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG\n    5451  GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA\n    5501  AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA\n    5551  AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG\n    5601  GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA\n    5651  AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA\n    5701  GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT\n    5751  ACAGGGCGCG TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG\n    5801  ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG\n    5851  CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT\n    5901  TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT\n    5951  GGGCCCGACG TCGCATGCTC CCGGCCGCCA TGGCGGCCGC GGGAATTCGA\n    6001  TATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACCATA TGGGAGAGCT\n    6051  CCCAACGCGT TGGATGCATA GCTTGAGTAT TCTATAGTGT CACCTAAATA\n    6101  GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG\n    6151  CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG\n    6201  GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCGACGCAA\n    6251  GGTCGTTAAC GGATCCACTA GTTCTAGAGC GGCCGGCCGC CACCGCGGCT\n    6301  AGATCTCTAT CACTGATAGG GAGATCTCCC TATAGTGAGT CGTATTAATC\n    6351  AGCCGGTGTG TTGCCAAAGA CATTCTGTAT AATTTTCCAA CTACCCTAAC\n    6401  CTGATTCACT CATCGTTGAG CATTTGTTCT TCTACTTTTT GACCGCAAGT\n    6451  GTCGTTATTA GTATTCGGTC CTGGGCTGCA GGCTGCTAAC AAAGCCCGAA\n    6501  AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC\n    6551  CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGAACT\n    6601  ATATCCGGAT AGGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG\n    6651  CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG\n    6701  GTCTTGAGGG GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATTGGGCCCC\n    6751  TGCAG<\/pre>\n","protected":false},"excerpt":{"rendered":"
in silico assembly, October 2005 Landmarks 0001-0592: (592) RRNA spacer targeting fragment Not \u201cI\u201d 0593-3116: (2524) Vector backbone (pUC\/pGEM) 3117-3412: (296) T. brucei actin mRNA processing 3\u2019 3413-4435: (1023) Hygromycin B phosphotransferase ORF 4436-4570: (135) T. brucei actin mRNA processing 5\u2019 4571-4865: (295) RRNA promoter 4866-5338: (473) T7 terminator x2 fragment 5339-5358: (20) T7 promoter 5359-5418: (60) Tet operator fragment 5419-6246: […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-336","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/336","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=336"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/336\/revisions"}],"predecessor-version":[{"id":579,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/336\/revisions\/579"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}