{"id":317,"date":"2023-10-16T09:22:38","date_gmt":"2023-10-16T08:22:38","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=317"},"modified":"2026-02-13T15:57:32","modified_gmt":"2026-02-13T15:57:32","slug":"pnathyg-c-terminal-in-situ-tagging-construct","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/pnathyg-c-terminal-in-situ-tagging-construct\/","title":{"rendered":"pNATHYG<\/sup> C-terminal in situ tagging construct"},"content":{"rendered":"\n

Derived from known sequence<\/p>\n\n\n\n

Landmarks<\/strong><\/td>Available expression cassettes<\/strong><\/td><\/tr>
<\/td><\/tr>
0001-1535: (1535)<\/td>eGFP<\/em> Expression cassette<\/strong><\/td>Sequence page<\/strong><\/td>Expression cassette<\/strong><\/td>Region replaced<\/strong><\/td><\/tr>
<\/td>Asc<\/em>I Hin<\/em>dIII \u2014 792bp stuffer \u2014 Xba<\/em>I \u2014 eGFP<\/em> \u2014 Avr<\/em>II TGA Bam<\/em>HI<\/td>C-terminal 12MYC<\/a><\/td>x12MYC<\/sup><\/td>Xba<\/em>I \u2013 Bam<\/em>HI<\/td><\/tr>
1536-2166: (631)<\/td>T. brucei<\/em> \u03b2\u03b1 tubulin mRNA processing 5\u2032<\/td><\/td><\/td><\/td><\/tr>
2180-3205: (1026)<\/td>Hygromycin-B phosphotransferase ORF<\/td><\/td><\/td><\/td><\/tr>
3209-3498: (290)<\/td>T. brucei<\/em> actin mRNA processing 3\u2032<\/td>Additional selectable markers<\/strong><\/td><\/td><\/tr>
3499-6034: (2536)<\/td>Vector backbone<\/td>BSD<\/em><\/a><\/td>xeGFP<\/sup><\/td>Kpn<\/em>I \u2013 Stu<\/em>I<\/td><\/tr>
<\/td><\/td>x12MYC<\/sup><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    1  GGCGCGCCAA GCTTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n   51  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n  801  NNNNNNTCTA GAGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC\n  851  CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT\n  901  CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC\n  951  ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC\n 1001  CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC\n 1051  AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC\n 1101  ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA\n 1151  GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT\n 1201  TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC\n 1251  AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT\n 1301  GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG\n 1351  ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC\n 1401  GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA\n 1451  GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA\n 1501  CTCTCGGCAT GGACGAGCTG TACAAGTGAG GATCCACGCG GACGGGGCAT\n 1551  TTCCCGTTCG TCATTAGCAG TAGGTAATGA AGATGTTTGT TTCTCGTCCC\n 1601  CTTTCTCCTT CGTCCTTCTG TCATTTTGTT CTTTTGTGTT TATGTTTTGT\n 1651  TGTTGTTTTC TTTAATTTTT TTTTTCTTCC ACGTTTGTGT ACATCCGCGC\n 1701  GCCACTCTAT TCAGAGAGCC ACGGATAGTA GAGGAGGTGG GAAGGGTATA\n 1751  TGAGGGACAC GCGTACCATG ATGTGGGATG TATTGGGGTC CCTGTCTGTC\n 1801  CTTACGTGAC TATGTATGAA CCGTCACGTG TAAGATGAGC TAGTGAGATC\n 1851  AACAGTACAA CTCATCAACA CGCCTTCTTC TCGTTAAATG TACACAATCT\n 1901  TGATCCTCCA CCTTTATGGG TCCCATTGTT TGCCTCTTCC GCTGTGTGGA\n 1951  GTGCGCCTAC ACGCACTTCT CACTTCGTAA GTGGTGGTGG CGTAAGTATT\n 2001  GCCTAATGTT GACTCTATAT TCTCCTCTCC TCACCCCCTC GCGGTGCTGA\n 2051  TTTCTGACAG ATCTTCAAAC ACTAGTTTAA GCAAAGGACT ATTCATCCGT\n 2101  TTATATTAGC AACAGTAGGT ACTAGCACCA CTAACAACAA CAACAAAGCA\n 2151  CTTCTATTTA TTTATCGGTA CCACTAGTGA TGAAAAAGCC TGAACTCACC\n 2201  GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA\n 2251  CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG\n 2301  TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC\n 2351  TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT\n 2401  TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA\n 2451  TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA\n 2501  CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC\n 2551  GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA\n 2601  TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC\n 2651  CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT\n 2701  CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG\n 2751  TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC\n 2801  AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA\n 2851  TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT\n 2901  GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA\n 2951  GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT\n 3001  CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG\n 3051  GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA\n 3101  CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT\n 3151  ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG\n 3201  AATAGAGGCC TCGGAGATCC TAACACCGGG TTGTGTGGCC AAAATTGTTC\n 3251  TGTAGTCGCT GTGAGTTGAC ACGGCTAGTG CTTATGATTT TCCTCGCGTG\n 3301  TGGTGCCTGT ACTCAGCCCT ATGCCTTATT TGCAACACAT TTACGTACAG\n 3351  CGCACAAGAG AAGAGAAGAT CACTTGAAGA TAATAAATAT AGGGTTGTAG\n 3401  GCATCTTGTT TAACTCAAAT TTTCTCGTCT TGGTGTGTCG ACATGATTGA\n 3451  AATAGTGCCA CCAGTTGTGT TTGATGCGTT TGTTATCTAT GCAGTATTCC\n 3501  TAGCCAGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA\n 3551  CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT\n 3601  CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT\n 3651  GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCACCATT\n 3701  CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC\n 3751  TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAATGGTGCA CTCTCAGTAC\n 3801  AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC\n 3851  CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA\n 3901  CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC\n 3951  ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT\n 4001  AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT\n 4051  CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC\n 4101  AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT\n 4151  ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT\n 4201  CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT\n 4251  GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA\n 4301  TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA\n 4351  GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT\n 4401  ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT\n 4451  ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT\n 4501  ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG\n 4551  TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG\n 4601  AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT\n 4651  CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC\n 4701  CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG\n 4751  AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG\n 4801  GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT\n 4851  TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG\n 4901  CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG\n 4951  ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT\n 5001  AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT\n 5051  ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG\n 5101  GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT\n 5151  TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT\n 5201  GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA\n 5251  CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT\n 5301  TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC\n 5351  TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT\n 5401  ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA\n 5451  TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG\n 5501  CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG\n 5551  CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG\n 5601  CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA\n 5651  GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG\n 5701  TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT\n 5751  TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG\n 5801  CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC\n 5851  TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA\n 5901  GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG\n 5951  TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG\n 6001  CGTTGGCCGA TTCATTAATG CAGAATTCGA GCTC<\/pre>\n","protected":false},"excerpt":{"rendered":"
Derived from known sequence Landmarks Available expression cassettes 0001-1535: (1535) eGFP Expression cassette Sequence page Expression cassette Region replaced AscI HindIII \u2014 792bp stuffer \u2014 XbaI \u2014 eGFP \u2014 AvrII TGA BamHI C-terminal 12MYC x12MYC XbaI \u2013 BamHI 1536-2166: (631) T. brucei \u03b2\u03b1 tubulin mRNA processing 5\u2032 2180-3205: (1026) Hygromycin-B phosphotransferase ORF 3209-3498: (290) T. brucei actin mRNA processing 3\u2032 Additional selectable markers 3499-6034: (2536) […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-317","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=317"}],"version-history":[{"count":4,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/317\/revisions"}],"predecessor-version":[{"id":582,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/317\/revisions\/582"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}