{"id":296,"date":"2023-10-13T16:39:22","date_gmt":"2023-10-13T15:39:22","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=296"},"modified":"2026-02-13T15:58:36","modified_gmt":"2026-02-13T15:58:36","slug":"pt7-sequence","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/pt7-sequence\/","title":{"rendered":"PT7 sequence"},"content":{"rendered":"\n
Landmarks<\/strong><\/td>Available expression cassettes<\/strong><\/td><\/tr>
<\/td><\/tr>
0001-0592: (592)<\/td>RRNA<\/em> targeting fragment Not<\/em>I<\/td>Sequence page<\/strong><\/td>Expression cassette<\/strong><\/td>Region replaced<\/strong><\/td><\/tr>
0593-3116: (2525)<\/td>Vector backbone (pUC\/pGEM)<\/td>N-term eGFP<\/a><\/td>GFP<\/sup>x<\/td>Hin<\/em>dIII \u2013 Bam<\/em>HI<\/td><\/tr>
3117-3412: (296)<\/td>T. brucei<\/em> actin mRNA processing 3\u2019<\/td>N-term MYC<\/a><\/td>MYC<\/sup>x<\/td><\/tr>
3413-4435: (1023)<\/td>Hygromycin B phosphotransferase ORF<\/td><\/td><\/td><\/td><\/tr>
4436-4571: (136)<\/td>T. brucei<\/em> actin mRNA processing 5\u2019<\/td><\/td><\/td><\/td><\/tr>
4572-4861: (290)<\/td>RRNA<\/em> promoter<\/td><\/td><\/td><\/td><\/tr>
4871-4890: (20)<\/td>T7 promoter<\/td><\/td><\/td><\/td><\/tr>
4899-4911: (13)<\/td>Tet operator<\/td><\/td><\/td><\/td><\/tr>
4922-5014: (93)<\/td>T. brucei<\/em> procyclin mRNA processing 5\u2032<\/td><\/td><\/td><\/td><\/tr>
5015-7549: (2535)<\/td>Expression Cassette<\/strong> (GFP<\/sup>x = 2,535 bp)<\/td><\/td><\/td><\/td><\/tr>
7550-8287: (738)<\/td>T. brucei<\/em> aldolase mRNA processing 3\u2019<\/td><\/td><\/td><\/td><\/tr>
8288-8689: (402)<\/td>T7 terminator x2 fragment<\/td><\/td><\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    1  GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT\n   51  CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT\n  101  TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT\n  151  AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA\n  201  CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA\n  251  TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG\n  301  GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT\n  351  GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA\n  401  GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG\n  451  GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC\n  501  TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA\n  551  GTCTGGAATT AGAATACATA CCAACTATAT GAGCTCGAAT TCTGCATTAA\n  601  TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC\n  651  CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG\n  701  CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG\n  751  GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA\n  801  CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG\n  851  ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA\n  901  GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC\n  951  TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT\n 1001  CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG\n 1051  GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA\n 1101  GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG\n 1151  TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC\n 1201  AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA\n 1251  CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC\n 1301  CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC\n 1351  ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG\n 1401  AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG\n 1451  CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA\n 1501  AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC\n 1551  AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA\n 1601  TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT\n 1651  GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC\n 1701  TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG\n 1751  ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT\n 1801  CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC\n 1851  TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG\n 1901  CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC\n 1951  TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA\n 2001  AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG\n 2051  CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT\n 2101  GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA\n 2151  GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT\n 2201  CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC\n 2251  ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT\n 2301  GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT\n 2351  CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT\n 2401  GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT\n 2451  CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA\n 2501  GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG\n 2551  CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT\n 2601  CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG\n 2651  CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG\n 2701  ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA\n 2751  GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG\n 2801  CATCAGAGCA GATTGTACTG AGAGTGCACC ATTCGACGCT CTCCCTTATG\n 2851  CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA\n 2901  CCGCCGCCGC AAGGAATGGT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG\n 2951  CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG\n 3001  AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC\n 3051  AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT\n 3101  AGAGGATCTG GCTAGGAATA CTGCATAGAT AACAAACGCA TCAAACACAA\n 3151  CTGGTGGCAC TATTTCAATC ATGTCGACAC ACCAAGACGA GAAAATTTGA\n 3201  GTTAAACAAG ATGCCTACAA CCCTATATTT ATTATCTTCA AGTGATCTTC\n 3251  TCTTCTCTTG TGCGCTGTAC GTAAATGTGT TGCAAATAAG GCATAGGGCT\n 3301  GAGTACAGGC ACCACACGCG AGGAAAATCA TAAGCACTAG CCGTGTCAAC\n 3351  TCACAGCGAC TACAGAACAA TTTTGGCCAC ACAACCCGGT GTTAGGATCT\n 3401  CCGAGGCCTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC\n 3451  CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT\n 3501  CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT\n 3551  TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA\n 3601  CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG\n 3651  AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT\n 3701  CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA\n 3751  CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT\n 3801  GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG\n 3851  CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT\n 3901  CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC\n 3951  CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT\n 4001  AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT\n 4051  GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC\n 4101  AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG\n 4151  TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT\n 4201  CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA\n 4251  TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA\n 4301  TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG\n 4351  AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC\n 4401  TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATCACTA GTTCTAGCTA\n 4451  GAGCTTATTT TATGGCAGCA ACGAGACCTT ACGTAAAGCA AAACTAAATA\n 4501  AGAGCGGAGA CTGCAATGCA GAGTAAAAAA AAAAAAAACA TGAATTTCAG\n 4551  AAGACCTTGC TGTGCCCGGG CTCTGAGAGC GGTCAGTTGC TCTCCTGCTC\n 4601  CGTACGCCGT AAGCGCTACT TTTACTGCTT AATCTTTCCT ATATAAGATA\n 4651  TATTTCTATT ACTATATATA CTTTTTTAAC TTTATCATTA TTATCCTATT\n 4701  GTGTGTTGTA TTGACTTTAC AATAAAACAT ATTATATCTA ACATGTCAAA\n 4751  AGCATCGGCT CTTCAATACA GTTACCCGTC CCTTATCAAC ACAGTACAAT\n 4801  ATACTCTGTC ATGACAATAA GTATATATAA GAGCCAGAAT GCACCCGCGC\n 4851  TGGGTGGAAA GCTAGCTGAT TAATACGACT CACTATAGGG AGATCTCCCT\n 4901  ATCAGTGATA GAGATCTAGC CCGGGCTGCA CGCGCCTTCG AGTTTTTTTT\n 4951  CCTTTTCCCC ATTTTTTTCA ACTTGAAGAC TTCAATTACA CCAAAAAGTA\n 5001  AAATTCACAA GCTTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 5951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 6951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN\n 7501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG\n 7551  GATCCTGCCC ATTTAGTTGG CTTTTCCCTT GTCTCGTGTC TTTTCCGTGG\n 7601  AAAGGTTCCC GGAGTAATCT GATGGCACAG CAGGGAGGTG CGCCTGCAGG\n 7651  TTGGTTAGGA AGGGGGGATG ATGTAAAAGA AGAAAATGGG GGGATATCTT\n 7701  ATGTTTAAGA GGATAAATAA TGTGAAGGGG CTTTATATGC TTGCTTGGTT\n 7751  GTCTCGTTGG TGCATGGGGA ATCTGCATGT TTGCTTTGGA GCACGCGTGG\n 7801  TACATCAATG GGATCATATC TTGCTGCCTC CCGCAGCTCA CCGTGCGAGC\n 7851  TGCCGGGACC CCGTTTCTCA TAGGCGTGTG CACCTTCCGT CTGAGACCTG\n 7901  TAAATGGTTA AAAAGGAATA TATAATTACC TTTTGAAAAG TGGTAAAAAA\n 7951  CGAATATATC TTTTTTTTTG GTTTTAATAC GCTCTTTTGT GTGTATGAGA\n 8001  GGAATAAATA AGTGTGTGTG TGTGTGTGTG TTGTTGTTGT TGAAAAAATG\n 8051  AAGGACACGA AAGCGCTCAT TAAGGGTGGG GATACACCAG GCATAATTAT\n 8101  CCGAAAATAT TTTGATGCAC CAAATAAGTG AACATACTGA CGAAATCAAA\n 8151  GAGTTGGAGG ATGGATAGGG AGGCCTCATT GGCAGTGGTA AATTGATTTG\n 8201  ACTTGAATAT GATACCCTCT TACTGTTTCA TTGCCACTAC AAGTTGGTTT\n 8251  CCTTCCCCTG CAGGCATGCA AGCTAGGCCA CACCTATCAG CCGGTGTGTT\n 8301  GCCAAAGACA TTCTGTATAA TTTTCCAACT ACCCTAACCT GATTCACTCA\n 8351  TCGTTGAGCA TTTGTTCTTC TACTTTTTGA CCGCAAGTGT CGTTATTAGT\n 8401  ATTCGGTCCT GGGCTGCAGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT\n 8451  TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT\n 8501  AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGAACTAT ATCCGGATAG\n 8551  GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG\n 8601  AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT\n 8651  TTTTTGCTGA AAGGAGAACT ATATCCGGAT TGGGCCCCTG CAG<\/pre>\n","protected":false},"excerpt":{"rendered":"
Landmarks Available expression cassettes 0001-0592: (592) RRNA targeting fragment NotI Sequence page Expression cassette Region replaced 0593-3116: (2525) Vector backbone (pUC\/pGEM) N-term eGFP GFPx HindIII \u2013 BamHI 3117-3412: (296) T. brucei actin mRNA processing 3\u2019 N-term MYC MYCx 3413-4435: (1023) Hygromycin B phosphotransferase ORF 4436-4571: (136) T. brucei actin mRNA processing 5\u2019 4572-4861: (290) RRNA promoter 4871-4890: (20) T7 promoter 4899-4911: […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-296","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/296","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=296"}],"version-history":[{"count":3,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/296\/revisions"}],"predecessor-version":[{"id":585,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/296\/revisions\/585"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=296"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}