{"id":272,"date":"2023-10-13T15:39:38","date_gmt":"2023-10-13T14:39:38","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=272"},"modified":"2026-02-13T15:58:18","modified_gmt":"2026-02-13T15:58:18","slug":"prpa-sequence","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/prpa-sequence\/","title":{"rendered":"pRPa sequence"},"content":{"rendered":"\n
Landmarks<\/strong><\/td>Available expression cassettes<\/strong><\/td><\/tr>
<\/td><\/tr>
0001-0600: (600)<\/td>RRNA<\/em> targeting fragment Asc<\/em>I<\/td>Sequence page<\/strong><\/td>Expression cassette<\/strong><\/td>Region replaced<\/strong><\/td><\/tr>
0601-3124: (2525)<\/td>Vector backbone (pUC\/pGEM)<\/td>Stem loop RNAi<\/a><\/td>MCS1\/2<\/td>Kpn<\/em>I \u2013 Bsp<\/em>120I<\/td><\/tr>
3125-3186: (62)<\/td>Remnant of T. brucei<\/em> actin mRNA processing 3\u2019<\/td>MCS3\/4<\/td><\/tr>
3187-4207: (1021)<\/td>Hygromycin B phosphotransferase ORF \u0394STOP Asc<\/em>I<\/td><\/tr>
4208-4465: (258)<\/td>T. brucei<\/em> aldolase mRNA processing 5\u2019<\/td>N-term eGFP<\/a><\/td>GFPx<\/td>Hin<\/em>dIII \u2013 Bam<\/em>HI<\/td><\/tr>
4449-4682: (234)<\/td>EP procyclin promoter<\/td>C-term eGFP<\/a><\/td>xGFP<\/td><\/tr>
4688-4947: (260)<\/td>RRNA promoter<\/td>N-term MYC<\/a><\/td>MYCx<\/td><\/tr>
4951-4963: (13)<\/td>Tet operator<\/td>N\/C-term 6MYC<\/a><\/td>x6MYCx<\/td><\/tr>
4970-5064: (95)<\/td>T. brucei<\/em> procyclin mRNA processing 5\u2032<\/td><\/td><\/td><\/td><\/tr>
5062-7596: (2535)<\/td>Expression Cassette<\/strong> (GFPx = 2,535 bp)<\/td>Constitutive<\/a><\/td>GFPx<\/td>Nhe<\/em>I \u2013 Kpn<\/em>I<\/td><\/tr>
7597-8334: (738)<\/td>T. brucei<\/em> aldolase mRNA processing 3\u2019<\/td><\/td><\/td><\/td><\/tr>
8335-8736: (402)<\/td>T7 terminator x2 fragment<\/td><\/td><\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    1  GAATTCGATG CGAGGCGAAT CGCTCAGTAG TGTTAGTGCA GGCATTGTTT
\n   51  CTCTTTTCCA TTTCGGCTCG CTCTCTGTGC CAGAAAGGCT TTTGGAGTCT
\n  101  TGGTTACTCG TGGGACTAGG GCAAGCGTCG CTTCCCTAAT AATTGTTGCT
\n  151  AACGTTTCAC CGTGCGTGTG TGGATATCTT CCAAGTTGTC ATTTTCTCTA
\n  201  CCCCTCTCTT TTCCGGCGCG GTCTAACTTC CTCCAACTAG CGCATTTCCA
\n  251  TGATAACGGG GTCCACATAG TCCGTTGTAG TGAAATTGGC ATGCCATCAG
\n  301  GGCCAAGGCA TACATCGCGG CCGCGATAAA AATGATACGA TGATCTCCGT
\n  351  GTGGAGCAGA CCGTGGCGAC CAGTTTGTAC CATGGAAGTT CTTCGATAGA
\n  401  GTTATATCGA GTGCGGAATT GATGCTACGG TCACAGTGTC GTATCGTGTG
\n  451  GTCTCCCATG TGTTTTTGAC GGTGTACCAA TTATCTTTGC ACTAATGGGC
\n  501  TATGGAATCT TTCCTTTTAA TCAGGGGGAC ATGTTTATCC CACGCCAGTA
\n  551  GTCTGGAATT AGAATACATA CCAACTATAT GGCGCGCCGA GCTCGAATTC
\n  601  TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
\n  651  CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
\n  701  GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
\n  751  AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
\n  801  GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
\n  851  CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
\n  901  ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
\n  951  GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
\n 1001  TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
\n 1051  TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
\n 1101  CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
\n 1151  CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
\n 1201  GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
\n 1251  TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
\n 1301  GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
\n 1351  AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
\n 1401  ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
\n 1451  GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
\n 1501  GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
\n 1551  TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
\n 1601  ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
\n 1651  CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
\n 1701  TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
\n 1751  GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
\n 1801  GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
\n 1851  CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
\n 1901  TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
\n 1951  CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
\n 2001  TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
\n 2051  TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
\n 2101  CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
\n 2151  TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
\n 2201  CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
\n 2251  TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
\n 2301  CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
\n 2351  TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
\n 2401  GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
\n 2451  CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
\n 2501  TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
\n 2551  GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC
\n 2601  ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
\n 2651  TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
\n 2701  TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
\n 2751  GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
\n 2801  CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT TCGACGCTCT
\n 2851  CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC
\n 2901  GTTGAGCACC GCCGCCGCAA GGAATGGTGC AAGGAGATGG CGCCCAACAG
\n 2951  TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA
\n 3001  TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA
\n 3051  TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG
\n 3101  TCCGGCGTAG AGGATCTGGC TAGGAATACT GCATAGATAA CAAACGCATC
\n 3151  AAACACAACT GGTGGCACTA TTTCAATCAT GTCGACGGCG CGCCCTCGGA
\n 3201  CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC
\n 3251  ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG
\n 3301  TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT
\n 3351  GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC
\n 3401  AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT
\n 3451  GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC
\n 3501  CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC
\n 3551  CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT
\n 3601  TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC
\n 3651  TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC
\n 3701  GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG
\n 3751  CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG
\n 3801  AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
\n 3851  CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA
\n 3901  GGTCTTGCGT GACACCCTGT GCACGGCGGG AGATGCAATA GGTCAGGCTC
\n 3951  TCGCTGAATT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA
\n 4001  TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC
\n 4051  TATTTACCCG CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA
\n 4101  CGAGATTCTT CGCCCTCCGA GAGCTGCATC AGGTCGGAGA CGCTGTCGAA
\n 4151  CTTTTCGATC AGAAACTTCT CGACAGACGT CGCGGTGAGT TCAGGCTTTT
\n 4201  TCATCACTAG ACTTCGTTGC AGTTGAGTTT CTGTTTTCGG GCTTGTTGGT
\n 4251  ATACTTGTTT GATTAATGTG GCTGATAGCT AAGCTATCGC AAATATTTGT
\n 4301  ACAAGACATG AGGGGGCAAA AAAATAAGCC AAGTGTATTT GTTAGGCTTC
\n 4351  CGTGCCTGTT AGAGAAATAT GTAAGGAAAC GCGTGCGCTA CACAGCTTGA
\n 4401  GCACCCATCG AATTGGGACG AGAACATGTT AAACTCAGTA CTCACCCGAT
\n 4451  AACCCCTATT TTCAATGCAC GCCCACCCAG AGACACGGAA TAGCATAAAA
\n 4501  CAACCCCATC ACTGCCACAA AATGGACATT TTGCACAAAA CGCACTATTG
\n 4551  ACTAGTATTC GACTATACTA ATAAGAGATA CCTATCAGAC ACGATATACT
\n 4601  GTGAAAAGGA CATTAAGCTC ACTATGCATC TGAAAACGCT TTCACCTCAC
\n 4651  AACCTACTTA CACACACCTT TCCGCTTAAC CCGCTAGCTT TCCACCCAGC
\n 4701  GCGGGTGCAT TCTGGCTCTT ATATATACTT ATTGTCATGA CAGAGTATAT
\n 4751  TGTACTGTGT TGATAAGGGA CGGGTAACTG TATTGAAGAG CCGATGCTTT
\n 4801  TGACATGTTA GATATAATAT GTTTTATTGT AAAGTCAATA CAACACACAA
\n 4851  TAGGATAATA ATGATAAAGT TAAAAAAGTA TATATAGTAA TAGAAATATA
\n 4901  TCTTATATAG GAAAGATTAA GCAGTAAAAG TAGCGCTTAC GGCGTACACC
\n 4951  CTATCAATGA TAGAGTGGTA CCCTGCACGC GCCTTCGAGT TTTTTTTCCT
\n 5001  TTTCCCCATT TTTTTCAACT TGAAGACTTC AATTACACCA AAAAGTAAAA
\n 5051  TTCACAAGCT TNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 5951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6601  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6651  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6701  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6751  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6801  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6851  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6901  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 6951  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7001  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7051  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7101  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7151  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7201  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7251  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7301  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7351  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7401  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7451  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7501  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
\n 7551  NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGAT
\n 7601  CCTGCCCATT TAGTTGGCTT TTCCCTTGTC TCGTGTCTTT TCCGTGGAAA
\n 7651  GGTTCCCGGA GTAATCTGAT GGCACAGCAG GGAGGTGCGC CTGCAGGTTG
\n 7701  GTTAGGAAGG GGGGATGATG TAAAAGAAGA AAATGGGGGG ATATCTTATG
\n 7751  TTTAAGAGGA TAAATAATGT GAAGGGGCTT TATATGCTTG CTTGGTTGTC
\n 7801  TCGTTGGTGC ATGGGGAATC TGCATGTTTG CTTTGGAGCA CGCGTGGTAC
\n 7851  ATCAATGGGA TCATATCTTG CTGCCTCCCG CAGCTCACCG TGCGAGCTGC
\n 7901  CGGGACCCCG TTTCTCATAG GCGTGTGCAC CTTCCGTCTG AGACCTGTAA
\n 7951  ATGGTTAAAA AGGAATATAT AATTACCTTT TGAAAAGTGG TAAAAAACGA
\n 8001  ATATATCTTT TTTTTTGGTT TTAATACGCT CTTTTGTGTG TATGAGAGGA
\n 8051  ATAAATAAGT GTGTGTGTGT GTGTGTGTTG TTGTTGTTGA AAAAATGAAG
\n 8101  GACACGAAAG CGCTCATTAA GGGTGGGGAT ACACCAGGCA TAATTATCCG
\n 8151  AAAATATTTT GATGCACCAA ATAAGTGAAC ATACTGACGA AATCAAAGAG
\n 8201  TTGGAGGATG GATAGGGAGG CCTCATTGGC AGTGGTAAAT TGATTTGACT
\n 8251  TGAATATGAT ACCCTCTTAC TGTTTCATTG CCACTACAAG TTGGTTTCCT
\n 8301  TCCCCTGCAG GCATGCAAGC TAGGCCACAC CTATCAGCCG GTGTGTTGCC
\n 8351  AAAGACATTC TGTATAATTT TCCAACTACC CTAACCTGAT TCACTCATCG
\n 8401  TTGAGCATTT GTTCTTCTAC TTTTTGACCG CAAGTGTCGT TATTAGTATT
\n 8451  CGGTCCTGGG CTGCAGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG
\n 8501  CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
\n 8551  CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GAACTATATC CGGATAGGGC
\n 8601  TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC
\n 8651  AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
\n 8701  TTGCTGAAAG GAGAACTATA TCCGGATTGG GCCCCTGCAG<\/pre>\n","protected":false},"excerpt":{"rendered":"
Landmarks Available expression cassettes 0001-0600: (600) RRNA targeting fragment AscI Sequence page Expression cassette Region replaced 0601-3124: (2525) Vector backbone (pUC\/pGEM) Stem loop RNAi MCS1\/2 KpnI \u2013 Bsp120I 3125-3186: (62) Remnant of T. brucei actin mRNA processing 3\u2019 MCS3\/4 3187-4207: (1021) Hygromycin B phosphotransferase ORF \u0394STOP AscI 4208-4465: (258) T. brucei aldolase mRNA processing 5\u2019 N-term eGFP GFPx HindIII \u2013 BamHI 4449-4682: (234) […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":202,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sub-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-272","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/272","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=272"}],"version-history":[{"count":10,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/272\/revisions"}],"predecessor-version":[{"id":584,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/272\/revisions\/584"}],"up":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}