{"id":202,"date":"2023-10-12T11:02:41","date_gmt":"2023-10-12T10:02:41","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/david-horn\/?page_id=202"},"modified":"2026-02-13T15:47:54","modified_gmt":"2026-02-13T15:47:54","slug":"resources","status":"publish","type":"page","link":"https:\/\/sites.dundee.ac.uk\/david-horn\/resources\/","title":{"rendered":"Resources"},"content":{"rendered":"\n

Tagging<\/h3>\n\n\n\n
Plasmid<\/th>Map (PDF)<\/th>Sequence<\/th>Description<\/th><\/tr><\/thead>
pRPaTAG<\/sup><\/td>pRPaTAG<\/sup> map<\/a><\/td>pRPa sequence<\/a><\/td>Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA<\/em> spacer locus in T. brucei<\/em>.Variants available (HYG<\/em>): pRPaiGFPx<\/sup>, pRPaixGFP<\/sup>, pRPaiMYCx<\/sup>, pRPaix6MYCx<\/sup>Constitutive expression plasmids, pRPaGFPx<\/sup> and pRPax6MYCx<\/sup>, are also available
pRPaTAG<\/sup> Details (PDF)<\/a><\/td><\/tr>
pRPTAG<\/sup><\/td>
pRPTAG<\/sup> map<\/a><\/td>
PRP sequence<\/a><\/td>		Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally tagged proteins from a random RRNA<\/em> spacer locus in T. brucei<\/em>.Variants available (HYG<\/em>): pRPiGFPx<\/sup>, pRPiMYCx<\/sup> (also BSD<\/em>), pRPix6MYCx<\/sup> (also BSD<\/em>)Constitutive expression plasmids (BSD<\/em>), pRPxGFP<\/sup> and pRPx6MYCx<\/sup>, are also available<\/td><\/tr>
pT7TAG<\/sup><\/td>
pT7TAG<\/sup> map<\/a><\/td>
pT7 sequence<\/a><\/td>		Tet-inducible T7 promoter driven plasmid for inducible expression of N-terminally tagged proteins from a random RRNA<\/em> spacer in T. brucei<\/em>.Variants available (HYG<\/em>): pT7iGFPx<\/sup>, pRPiMYCx<\/sup>pT7TAG<\/sup> Details (PDF)<\/a><\/td><\/tr>
pNATTAG<\/sup>x<\/td>pNATTAG<\/sup>x map<\/a><\/td>pNATTAG<\/sup>x sequence<\/a><\/td>A plasmid for expression of N-terminally tagged proteins from a gene\u2019s native locus.Variants available (BSD<\/em> and HYG<\/em>): pNATGFPx<\/sup>, pNAT6MYCx<\/sup><\/td><\/tr>
pNATxTAG<\/sup><\/td>pNATxTAG<\/sup> map<\/a><\/td>pNATxTAG<\/sup> sequence<\/a><\/a><\/td>A plasmid for expression of C-terminally tagged proteins from a gene\u2019s native locus.Variants available (BSD<\/em> and HYG<\/em>): pNATxGFP<\/sup>, pNATx12MYC<\/sup><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

RNAi<\/h2>\n\n\n\n
Plasmid<\/th>Map (PDF)<\/th>Sequence<\/th>Description<\/th><\/tr><\/thead>
pRPaiSL<\/sup><\/td>pRPaSL map<\/a><\/td>pRPa sequence<\/a><\/td>Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.pRPaSL Details (PDF)<\/a><\/td><\/tr>
p2T7TAblue<\/sup><\/td>p2T7TAblue<\/sup> map<\/a><\/td>p2T7TAblue<\/sup> sequence<\/a><\/td>Head-to-head T7 promoter-driven plasmid for the generation of dsRNA for RNAi experiments in T. brucei.p2T7aTAblue (sequence<\/a>) is also available \u2013 compatible with the tagged locus cell line when modified to express T7 polymerase.Details (PDF)<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Plasmids<\/strong><\/h3>\n\n\n\n

Several of these constructs are also available from Addgene<\/a>.<\/p>\n\n\n\n

View inducible expression data<\/a>.<\/p>\n\n\n\n

For more information on cells and vectors see: Inducible cell lines<\/a> (word document), and George Cross<\/a> and Christine Clayton\u2019s<\/a> web-sites.<\/p>\n","protected":false},"excerpt":{"rendered":"

Tagging Plasmid Map (PDF) Sequence Description pRPaTAG pRPaTAG map pRPa sequence Tet-inducible RRNA promoter driven plasmid for expression of N- or C-terminally tagged proteins from a specific RRNA spacer locus in T. brucei.Variants available (HYG): pRPaiGFPx, pRPaixGFP, pRPaiMYCx, pRPaix6MYCxConstitutive expression plasmids, pRPaGFPx and pRPax6MYCx, are also availablepRPaTAG Details (PDF) pRPTAG pRPTAG map PRP sequence Tet-inducible RRNA promoter driven plasmid for inducible expression […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-landing.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-202","page","type-page","status-publish","hentry"],"blocksy_meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/comments?post=202"}],"version-history":[{"count":33,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202\/revisions"}],"predecessor-version":[{"id":564,"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/pages\/202\/revisions\/564"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/david-horn\/wp-json\/wp\/v2\/media?parent=202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}