{"id":2821,"date":"2014-09-30T08:42:00","date_gmt":"2014-09-30T07:42:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2821"},"modified":"2026-01-26T16:28:06","modified_gmt":"2026-01-26T16:28:06","slug":"elucidating-binding-hotspots-of-the-baz2b-bromodomainhistone-interaction","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2014\/09\/30\/elucidating-binding-hotspots-of-the-baz2b-bromodomainhistone-interaction\/","title":{"rendered":"Elucidating binding hotspots of the BAZ2B bromodomain:histone interaction"},"content":{"rendered":"<p>Our paper elucidating binding hotspots of the BAZ2B bromodomain:histone interaction now published in Biochemistry.<\/p>\n<p><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/bi500909d\">Read our paper on ACS publication website<\/a>.<\/p>\n<div id='gallery-1' class='gallery galleryid-2821 gallery-columns-3 gallery-size-large'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9.png'><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"729\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9.png\" class=\"attachment-large size-large\" alt=\"Figure 9. Views of the HADDOCK model\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1008\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8.png\" class=\"attachment-large size-large\" alt=\"Figure 8. Results of HADDOCK docking\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7.png'><img loading=\"lazy\" decoding=\"async\" width=\"901\" height=\"841\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7.png\" class=\"attachment-large size-large\" alt=\"Figure 7. CLEANEX-PM spectra of BAZ2B bromodomain\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-scaled.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"862\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-scaled.png\" class=\"attachment-large size-large\" alt=\"Figure 6. Peptide HSQC titration\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0.png'><img loading=\"lazy\" decoding=\"async\" width=\"985\" height=\"508\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0.png\" class=\"attachment-large size-large\" alt=\"Figure 5. Small molecule HSQC titration\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_.png'><img loading=\"lazy\" decoding=\"async\" width=\"862\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_.png\" class=\"attachment-large size-large\" alt=\"Figure 4. Dynamics of the BAZ2B bromodomain\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"668\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Figure 3. Parameters describing internal dynamics for the assigned and nonoverlapping residues of BAZ2B bromodomain\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"753\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Figure 2. BAZ2B spectral assignment\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-scaled.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"718\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-scaled.png\" class=\"attachment-large size-large\" alt=\"Figure 1. Structural features of the BAZ2B bromodomain\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-scaled.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"407\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-scaled.jpeg\" class=\"attachment-large size-large\" alt=\"Graphic for elucidating binding hotspots of the BAZ2B bromodomain:histone interaction\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Our paper elucidating binding hotspots of the BAZ2B bromodomain:histone interaction now published in Biochemistry. Read our paper on ACS publication website.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22,4],"tags":[],"class_list":["post-2821","post","type-post","status-publish","format-standard","hentry","category-22","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2821"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821\/revisions"}],"predecessor-version":[{"id":4887,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821\/revisions\/4887"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}