{"id":2821,"date":"2014-09-30T08:42:00","date_gmt":"2014-09-30T07:42:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2821"},"modified":"2026-01-26T16:28:06","modified_gmt":"2026-01-26T16:28:06","slug":"elucidating-binding-hotspots-of-the-baz2b-bromodomainhistone-interaction","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2014\/09\/30\/elucidating-binding-hotspots-of-the-baz2b-bromodomainhistone-interaction\/","title":{"rendered":"Elucidating binding hotspots of the BAZ2B bromodomain:histone interaction"},"content":{"rendered":"<p>Our paper elucidating binding hotspots of the BAZ2B bromodomain:histone interaction now published in Biochemistry.<\/p>\n<p><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/bi500909d\">Read our paper on ACS publication website<\/a>.<\/p>\n<div id='gallery-1' class='gallery galleryid-2821 gallery-columns-3 gallery-size-large'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9.png'><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"729\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9.png\" class=\"attachment-large size-large\" alt=\"Figure 9. Views of the HADDOCK model\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9.png 900w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9-300x243.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig9-768x622.png 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1008\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8-1024x1008.png\" class=\"attachment-large size-large\" alt=\"Figure 8. Results of HADDOCK docking\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8-1024x1008.png 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8-300x295.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8-768x756.png 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig8.png 1114w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7.png'><img loading=\"lazy\" decoding=\"async\" width=\"901\" height=\"841\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7.png\" class=\"attachment-large size-large\" alt=\"Figure 7. CLEANEX-PM spectra of BAZ2B bromodomain\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7.png 901w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7-300x280.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig7-revised-Figure-7-768x717.png 768w\" sizes=\"auto, (max-width: 901px) 100vw, 901px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"861\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-1024x861.png\" class=\"attachment-large size-large\" alt=\"Figure 6. Peptide HSQC titration\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-1024x861.png 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-300x252.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6-768x646.png 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig6.png 1260w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0.png'><img loading=\"lazy\" decoding=\"async\" width=\"985\" height=\"508\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0.png\" class=\"attachment-large size-large\" alt=\"Figure 5. Small molecule HSQC titration\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0.png 985w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0-300x155.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig5-Fig5_0-768x396.png 768w\" sizes=\"auto, (max-width: 985px) 100vw, 985px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_.png'><img loading=\"lazy\" decoding=\"async\" width=\"862\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_-862x1024.png\" class=\"attachment-large size-large\" alt=\"Figure 4. Dynamics of the BAZ2B bromodomain\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_-862x1024.png 862w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_-253x300.png 253w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_-768x912.png 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig4-Updated-Figure-4_.png 872w\" sizes=\"auto, (max-width: 862px) 100vw, 862px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"668\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-668x1024.jpg\" class=\"attachment-large size-large\" alt=\"Figure 3. Parameters describing internal dynamics for the assigned and nonoverlapping residues of BAZ2B bromodomain\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-668x1024.jpg 668w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-196x300.jpg 196w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-768x1177.jpg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-1002x1536.jpg 1002w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003-1336x2048.jpg 1336w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig3-master.img-003.jpg 2000w\" sizes=\"auto, (max-width: 668px) 100vw, 668px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"753\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-753x1024.jpg\" class=\"attachment-large size-large\" alt=\"Figure 2. BAZ2B spectral assignment\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-753x1024.jpg 753w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-221x300.jpg 221w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002-768x1044.jpg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig2-master.img-002.jpg 1000w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0.png'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"718\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-1024x718.png\" class=\"attachment-large size-large\" alt=\"Figure 1. Structural features of the BAZ2B bromodomain\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-1024x718.png 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-300x210.png 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0-768x538.png 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1a-Fig1_0.png 1420w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"407\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-1024x407.jpeg\" class=\"attachment-large size-large\" alt=\"Graphic for elucidating binding hotspots of the BAZ2B bromodomain:histone interaction\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-1024x407.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-300x119.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011-768x305.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/elucidating-binding-hotspots-fig1-bi-2014-00909d_0011.jpeg 1375w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Our paper elucidating binding hotspots of the BAZ2B bromodomain:histone interaction now published in Biochemistry. Read our paper on ACS publication website.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22,4],"tags":[],"class_list":["post-2821","post","type-post","status-publish","format-standard","hentry","category-22","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2821"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821\/revisions"}],"predecessor-version":[{"id":4887,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2821\/revisions\/4887"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}