{"id":2761,"date":"2014-11-12T15:34:00","date_gmt":"2014-11-12T15:34:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2761"},"modified":"2026-01-26T16:28:06","modified_gmt":"2026-01-26T16:28:06","slug":"biophysical-investigation-of-interactions-and-assembly-of-full-size-socs2elobccul5rbx2-e3-ubiquitin-ligase","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2014\/11\/12\/biophysical-investigation-of-interactions-and-assembly-of-full-size-socs2elobccul5rbx2-e3-ubiquitin-ligase\/","title":{"rendered":"Biophysical investigation of interactions and assembly of full-size SOCS2:EloBC:Cul5:Rbx2 E3 ubiquitin ligase"},"content":{"rendered":"<p>Our paper on biophysical investigation of interactions and assembly of full-size SOCS2:EloBC:Cul5:Rbx2 E3 ubiquitin ligase is now published in J. Biol. Chem.<\/p>\n<p><a href=\"http:\/\/dx.doi.org\/10.1074\/jbc.M114.616664\">Read our\u00a0Just In Press article<\/a>.<\/p>\n<div id='gallery-1' class='gallery galleryid-2761 gallery-columns-3 gallery-size-large'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-JBC_front-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"574\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-JBC_front-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Our paper on biophysical investigation of interactions and assembly of full-size SOCS2:EloBC:Cul5:Rbx2 E3 ubiquitin ligase is now published in J. Biol. Chem.\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-1-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"696\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-1-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 1. Components of CRL5SOCS2 E3 ligase can be captured from cell lysate using phosphorylated substrate peptide attached to the beads\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-2-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"543\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-2-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 2. ITC data demonstrates weak interaction of SOCS2-EloBC with phosphorylated substrate GHR and tight interaction with scaffold Cul5NTD\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-3_-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"673\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-3_-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 3. Recombinant components of CRL5SOCS2 assemble into the monomeric full-size protein complex\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-4_-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"693\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-4_-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 4. Ion mobility drift-time plot (top), corresponding native mass spectra (middle) and collision cross sections (bottom) are shown for the CRL5SOCS2 complexes and their components.\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-5_-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"236\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-5_-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 5. Experimental ion mobility data for a range of charge states suggests an increase in CCS values upon neddylation of the protein complexes.\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-6-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"976\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-6-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 6. Gel filtration UV traces\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-7_-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"839\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-7_-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 7. Native MS spectra\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-8-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"688\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-8-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 8. SDS-PAGE gel images of the neddylation reaction products.\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-9_-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"449\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-9_-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 9. Comparison of Native MS spectra.\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-10_scaled-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"860\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/SOCS2-EloBC-Cul5-Rbx2-Figure-10_scaled-scaled.jpg\" class=\"attachment-large size-large\" alt=\"Fig. 10. Structural models provide important insights into the assembly and architecture of CRL5SOCS2\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Our paper on biophysical investigation of interactions and assembly of full-size SOCS2:EloBC:Cul5:Rbx2 E3 ubiquitin ligase is now published in J. Biol. Chem. Read our\u00a0Just In Press article.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22,4],"tags":[],"class_list":["post-2761","post","type-post","status-publish","format-standard","hentry","category-22","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2761"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2761\/revisions"}],"predecessor-version":[{"id":4884,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2761\/revisions\/4884"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2761"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2761"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}