{"id":2495,"date":"2013-03-11T08:17:00","date_gmt":"2013-03-11T08:17:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2495"},"modified":"2026-01-26T16:28:06","modified_gmt":"2026-01-26T16:28:06","slug":"our-paper-interrogating-the-nmr-binding-detection-limits-of-fragments-targeting-the-vhl-hif1%ce%b1-ppi","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2013\/03\/11\/our-paper-interrogating-the-nmr-binding-detection-limits-of-fragments-targeting-the-vhl-hif1%ce%b1-ppi\/","title":{"rendered":"Our paper interrogating the NMR binding detection limits of fragments targeting the VHL-HIF1\u03b1 PPI"},"content":{"rendered":"<p>Read our paper published in ACS Med. Chem. Lett. about  interrogating the NMR binding detection limits of fragments targeting the VHL-HIF1\u03b1 PPI.<\/p>\n<\/p>\n<p><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/ml400296c\">You can read our open-access paper<\/a>.<\/p>\n<div id='gallery-1' class='gallery galleryid-2495 gallery-columns-3 gallery-size-large'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-1.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"399\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-1.jpeg\" class=\"attachment-large size-large\" alt=\"Table of content graphic for NMR binding detection\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-1.jpeg 1000w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-1-300x120.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-1-768x306.jpeg 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-2.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"933\" height=\"643\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-2.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 1. Structural representation of the VCB multiprotein complex and the pVHL:HIF-1\u03b1 interface\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-2.jpeg 933w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-2-300x207.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-2-768x529.jpeg 768w\" sizes=\"auto, (max-width: 933px) 100vw, 933px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"823\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3-823x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 2. Binding detection at the pVHL:HIF-1\u03b1 interface for fragment 4.\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3-823x1024.jpeg 823w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3-241x300.jpeg 241w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3-768x955.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-3.jpeg 992w\" sizes=\"auto, (max-width: 823px) 100vw, 823px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"685\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4-685x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 3. Detection of capped-Hydroxyproline 6 binding to VCB using ligand-based NMR spectroscopy\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4-685x1024.jpeg 685w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4-201x300.jpeg 201w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4-768x1148.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-4.jpeg 858w\" sizes=\"auto, (max-width: 685px) 100vw, 685px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"685\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5-685x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 4. Group epitope mapping (GEM) obtained from STD-NMR for 6.\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5-685x1024.jpeg 685w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5-201x300.jpeg 201w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5-768x1148.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-5.jpeg 858w\" sizes=\"auto, (max-width: 685px) 100vw, 685px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-6.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"425\" height=\"314\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-6.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 5. Residue specific mapping of the binding site of fragment 8 using 1H\u221215N HSQC\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-6.jpeg 425w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/NMR-binding-detection-6-300x222.jpeg 300w\" sizes=\"auto, (max-width: 425px) 100vw, 425px\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Read our paper published in ACS Med. Chem. Lett. about interrogating the NMR binding detection limits of fragments targeting the VHL-HIF1\u03b1 PPI. You can read our open-access paper.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16,4],"tags":[],"class_list":["post-2495","post","type-post","status-publish","format-standard","hentry","category-16","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2495","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2495"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2495\/revisions"}],"predecessor-version":[{"id":4892,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2495\/revisions\/4892"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2495"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2495"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2495"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}