{"id":2429,"date":"2019-05-02T15:25:00","date_gmt":"2019-05-02T14:25:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2429"},"modified":"2026-01-26T16:28:04","modified_gmt":"2026-01-26T16:28:04","slug":"our-new-paper-describing-an-spr-kinetic-assay-to-monitor-protac-ternary-complexes-is-now-out-in-acs-chemical-biology","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2019\/05\/02\/our-new-paper-describing-an-spr-kinetic-assay-to-monitor-protac-ternary-complexes-is-now-out-in-acs-chemical-biology\/","title":{"rendered":"Our new paper describing an SPR kinetic assay to monitor PROTAC ternary complexes is now out in ACS Chemical Biology"},"content":{"rendered":"<p>Measuring kinetics by surface plasmon resonance (SPR), we reveal how striking differences in stability and lifetime of target:PROTAC:ligase ternary complexes influence initial rates of PROTAC induced protein degradation. Congratulations to Michael, Scott, Claire, Will and our colleagues at Boehringer Ingelheim.\u00a0<a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acschembio.9b00092\">Read more about our paper<\/a>.<\/p>\n<ul>\n<li>Pre-print of this paper was first posted at bioRxiv 451948 on 25 October 2018 doi:\u00a0<a href=\"https:\/\/doi.org\/10.1101\/451948\">https:\/\/doi.org\/10.1101\/451948<\/a><\/li>\n<li>Selected to be featured in\u00a0<a href=\"http:\/\/www.pubs.acs.org\/editorschoice\">ACS Editors\u2019 Choice<\/a>\u00a0based on recommendation by ACS journals\u2019 editors.\u00a0Under ACS Editors\u2019 Choice, our article has been sponsored for immediate, free open access by ACS due to its potential for broad public interest, an honor given to only one article from the entire ACS portfolio each day of the year.\u00a0<\/li>\n<li>Highlighted in Derek Lowe\u2019s blog\u00a0<a href=\"https:\/\/blogs.sciencemag.org\/pipeline\/archives\/2019\/03\/12\/what-those-degraders-are-actually-doing\">In the Pipeline<\/a><\/li>\n<\/ul>\n<div id='gallery-1' class='gallery galleryid-2429 gallery-columns-3 gallery-size-large'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-1.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"502\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-1.jpeg\" class=\"attachment-large size-large\" alt=\"ToC Graphic for SPR kinetic assay to monitor PROTAC ternary complexes\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-1.jpeg 1000w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-1-300x151.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-1-768x386.jpeg 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"757\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2-757x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 1. Schematic and binding data illustrating our SPR approach for measuring binding kinetics and determining cooperativity (\u03b1) for PROTAC binary and ternary complex formation\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2-757x1024.jpeg 757w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2-222x300.jpeg 222w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2-768x1039.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-2.jpeg 1000w\" sizes=\"auto, (max-width: 757px) 100vw, 757px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"690\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3-1024x690.jpeg\" class=\"attachment-large size-large\" alt=\"Chart 1. PROTACs Utilized in This Study\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3-1024x690.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3-300x202.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3-768x517.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3-1536x1034.jpeg 1536w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-3.jpeg 2000w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"481\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-1024x481.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 3\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-1024x481.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-300x141.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-768x361.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-1536x722.jpeg 1536w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-4-2048x963.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"671\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5-671x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 4\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5-671x1024.jpeg 671w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5-196x300.jpeg 196w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5-768x1173.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-5.jpeg 1000w\" sizes=\"auto, (max-width: 671px) 100vw, 671px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6.jpeg'><img loading=\"lazy\" decoding=\"async\" width=\"677\" height=\"1024\" src=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6-677x1024.jpeg\" class=\"attachment-large size-large\" alt=\"Figure 5\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6-677x1024.jpeg 677w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6-198x300.jpeg 198w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6-768x1161.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/protac-ternary-6.jpeg 1000w\" sizes=\"auto, (max-width: 677px) 100vw, 677px\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Measuring kinetics by surface plasmon resonance (SPR), we reveal how striking differences in stability and lifetime of target:PROTAC:ligase ternary complexes influence initial rates of PROTAC induced protein degradation. Congratulations to Michael, Scott, Claire, Will and our colleagues at Boehringer Ingelheim.\u00a0Read more about our paper. Pre-print of this paper was first posted at bioRxiv 451948 on [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,4],"tags":[],"class_list":["post-2429","post","type-post","status-publish","format-standard","hentry","category-17","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2429","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2429"}],"version-history":[{"count":2,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2429\/revisions"}],"predecessor-version":[{"id":4862,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2429\/revisions\/4862"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2429"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2429"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2429"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}