{"id":2382,"date":"2019-04-12T14:23:00","date_gmt":"2019-04-12T13:23:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2382"},"modified":"2026-01-28T10:00:18","modified_gmt":"2026-01-28T10:00:18","slug":"our-collaboration-with-dario-alessis-lab-on-deploying-improved-haloprotacs-against-crispred-halo-tagged-endosomal-proteins-is-now-out-in-acs-chem-biol","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2019\/04\/12\/our-collaboration-with-dario-alessis-lab-on-deploying-improved-haloprotacs-against-crispred-halo-tagged-endosomal-proteins-is-now-out-in-acs-chem-biol\/","title":{"rendered":"Our collaboration with Dario Alessi&#8217;s Lab on deploying improved HaloPROTACs against CRISPR&#8217;ed halo-tagged endosomal proteins is now out in ACS Chem Biol"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1021\/acschembio.8b01016\">Read the open access article<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Congratulations to&nbsp;<a href=\"https:\/\/www.ppu.mrc.ac.uk\/about-us\/phd-and-postdoc-profiles\/hannah-tovell\">Hannah Tovell<\/a>&nbsp;in Dario Alessi\u2019s group for the excellent job on this study. Well done Andrea and Chiara for the chemistry contributions to the project!<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"999\" height=\"470\" data-id=\"2391\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1.jpeg\" alt=\"TOC graphic for haloPROTACS\" class=\"wp-image-2391\"\/><\/a><figcaption class=\"wp-element-caption\">TOC graphic for haloPROTACS<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2.jpeg\"><img decoding=\"async\" data-id=\"2384\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2-1024x845.jpeg\" alt=\"Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR\/Cas9\" class=\"wp-image-2384\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR\/Cas9<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3.jpeg\"><img decoding=\"async\" data-id=\"2385\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-730x1024.jpeg\" alt=\"Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7\" class=\"wp-image-2385\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4.jpeg\"><img decoding=\"async\" data-id=\"2383\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-1024x490.jpeg\" alt=\"Figure 3. Chemical structures of HaloPROTAC3 and Compounds A\u2013F\" class=\"wp-image-2383\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 3. Chemical structures of HaloPROTAC3 and Compounds A\u2013F<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5.jpeg\"><img decoding=\"async\" data-id=\"2386\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-1024x995.jpeg\" alt=\"Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins\" class=\"wp-image-2386\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6.jpeg\"><img decoding=\"async\" data-id=\"2387\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-777x1024.jpeg\" alt=\"Figure 5. Mechanistic characterization of HaloPROTAC-E\" class=\"wp-image-2387\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 5. Mechanistic characterization of HaloPROTAC-E<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7.jpeg\"><img decoding=\"async\" data-id=\"2388\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-1024x566.jpeg\" alt=\"Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3\" class=\"wp-image-2388\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8.jpeg\"><img decoding=\"async\" data-id=\"2389\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-620x1024.jpeg\" alt=\"Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins\" class=\"wp-image-2389\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9.jpeg\"><img decoding=\"async\" data-id=\"2390\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9-1024x746.jpeg\" alt=\"Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact\" class=\"wp-image-2390\"\/><\/a><figcaption class=\"wp-element-caption\">Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact<\/figcaption><\/figure>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Read the open access article Congratulations to&nbsp;Hannah Tovell&nbsp;in Dario Alessi\u2019s group for the excellent job on this study. Well done Andrea and Chiara for the chemistry contributions to the project!<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,4],"tags":[],"class_list":["post-2382","post","type-post","status-publish","format-standard","hentry","category-17","category-news"],"blocksy_meta":{"styles_descriptor":{"styles":{"desktop":"","tablet":"","mobile":""},"google_fonts":[],"version":7}},"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2382"}],"version-history":[{"count":4,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382\/revisions"}],"predecessor-version":[{"id":4957,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382\/revisions\/4957"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2382"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2382"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2382"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}