{"id":2382,"date":"2019-04-12T14:23:00","date_gmt":"2019-04-12T13:23:00","guid":{"rendered":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/?p=2382"},"modified":"2026-01-28T10:00:18","modified_gmt":"2026-01-28T10:00:18","slug":"our-collaboration-with-dario-alessis-lab-on-deploying-improved-haloprotacs-against-crispred-halo-tagged-endosomal-proteins-is-now-out-in-acs-chem-biol","status":"publish","type":"post","link":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/2019\/04\/12\/our-collaboration-with-dario-alessis-lab-on-deploying-improved-haloprotacs-against-crispred-halo-tagged-endosomal-proteins-is-now-out-in-acs-chem-biol\/","title":{"rendered":"Our collaboration with Dario Alessi&#8217;s Lab on deploying improved HaloPROTACs against CRISPR&#8217;ed halo-tagged endosomal proteins is now out in ACS Chem Biol"},"content":{"rendered":"\n<p><a href=\"https:\/\/doi.org\/10.1021\/acschembio.8b01016\">Read the open access article<\/a><\/p>\n\n\n\n<p>Congratulations to&nbsp;<a href=\"https:\/\/www.ppu.mrc.ac.uk\/about-us\/phd-and-postdoc-profiles\/hannah-tovell\">Hannah Tovell<\/a>&nbsp;in Dario Alessi\u2019s group for the excellent job on this study. Well done Andrea and Chiara for the chemistry contributions to the project!<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"999\" height=\"470\" data-id=\"2391\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1.jpeg\" alt=\"TOC graphic for haloPROTACS\" class=\"wp-image-2391\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1.jpeg 999w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1-300x141.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-1-768x361.jpeg 768w\" sizes=\"auto, (max-width: 999px) 100vw, 999px\" \/><\/a><figcaption class=\"wp-element-caption\">TOC graphic for haloPROTACS<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"845\" data-id=\"2384\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2-1024x845.jpeg\" alt=\"Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR\/Cas9\" class=\"wp-image-2384\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2-1024x845.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2-300x247.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2-768x633.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-2.jpeg 1364w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 1. Generation of HaloTag7 endogenous fusion proteins by CRISPR\/Cas9<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"730\" height=\"1024\" data-id=\"2385\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-730x1024.jpeg\" alt=\"Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7\" class=\"wp-image-2385\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-730x1024.jpeg 730w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-214x300.jpeg 214w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-768x1077.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-1096x1536.jpeg 1096w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3-1461x2048.jpeg 1461w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-3.jpeg 1542w\" sizes=\"auto, (max-width: 730px) 100vw, 730px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 2. Localization and function of VPS34 and SGK3 are unaffected by fusion to HaloTag7<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"490\" data-id=\"2383\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-1024x490.jpeg\" alt=\"Figure 3. Chemical structures of HaloPROTAC3 and Compounds A\u2013F\" class=\"wp-image-2383\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-1024x490.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-300x144.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-768x367.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-1536x735.jpeg 1536w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-4-2048x980.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 3. Chemical structures of HaloPROTAC3 and Compounds A\u2013F<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"995\" data-id=\"2386\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-1024x995.jpeg\" alt=\"Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins\" class=\"wp-image-2386\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-1024x995.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-300x292.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-768x746.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5-1536x1493.jpeg 1536w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-5.jpeg 1749w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 4. HaloPROTAC-mediated degradation of HaloTag7-fusion proteins<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"777\" height=\"1024\" data-id=\"2387\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-777x1024.jpeg\" alt=\"Figure 5. Mechanistic characterization of HaloPROTAC-E\" class=\"wp-image-2387\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-777x1024.jpeg 777w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-228x300.jpeg 228w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-768x1012.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-1166x1536.jpeg 1166w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-6-1554x2048.jpeg 1554w\" sizes=\"auto, (max-width: 777px) 100vw, 777px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 5. Mechanistic characterization of HaloPROTAC-E<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"566\" data-id=\"2388\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-1024x566.jpeg\" alt=\"Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3\" class=\"wp-image-2388\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-1024x566.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-300x166.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-768x424.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7-1536x848.jpeg 1536w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-7.jpeg 1606w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 6. Comparison of HaloPROTAC-E to previously reported HaloPROTAC3<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"620\" height=\"1024\" data-id=\"2389\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-620x1024.jpeg\" alt=\"Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins\" class=\"wp-image-2389\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-620x1024.jpeg 620w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-182x300.jpeg 182w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-768x1269.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-930x1536.jpeg 930w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8-1240x2048.jpeg 1240w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-8.jpeg 1270w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 7. HaloPROTAC-E induces degradation of endosomally localized proteins<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"746\" data-id=\"2390\" src=\"https:\/\/sites.dundee.ac.uk\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9-1024x746.jpeg\" alt=\"Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact\" class=\"wp-image-2390\" srcset=\"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9-1024x746.jpeg 1024w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9-300x219.jpeg 300w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9-768x560.jpeg 768w, https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-content\/uploads\/sites\/233\/2024\/01\/haloPROTACS-9.jpeg 1376w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 8. Degradation of HaloTag7 fusions is highly specific and has a biological impact<\/figcaption><\/figure>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Read the open access article Congratulations to&nbsp;Hannah Tovell&nbsp;in Dario Alessi\u2019s group for the excellent job on this study. Well done Andrea and Chiara for the chemistry contributions to the project!<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,4],"tags":[],"class_list":["post-2382","post","type-post","status-publish","format-standard","hentry","category-17","category-news"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/comments?post=2382"}],"version-history":[{"count":4,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382\/revisions"}],"predecessor-version":[{"id":4957,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/posts\/2382\/revisions\/4957"}],"wp:attachment":[{"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/media?parent=2382"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/categories?post=2382"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.dundee.ac.uk\/alessio-ciulli\/wp-json\/wp\/v2\/tags?post=2382"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}